Huang Yali, Dong Yan, Ren Yachao, Wang Shijie, Li Yongtan, Du Kejiu, Lin Xin, Yang Minsheng
Institute of Forest Biotechnology, Forestry College, Hebei Agricultural University, Baoding, China.
Hebei Key Laboratory for Tree Genetic Resources and Forest Protection, Baoding, China.
Front Microbiol. 2022 Jan 28;12:805261. doi: 10.3389/fmicb.2021.805261. eCollection 2021.
Exploring the complex spatiotemporal changes and colonization mechanism of microbial communities will enable microbial communities to be better used to serve agricultural and ecological operations. In addition, evaluating the impact of transgenic plants on endogenous microbial communities is necessary for their commercial application. In this study, microbial communities of × 'Neva' carrying c-Cry3A-BADH genes (ECAA1 line), × 'Neva' carrying genes (ECAB1 line), and non-transgenic × 'Neva' from rhizosphere soil, roots, and phloem collected in different seasons were compared and analyzed. Our analyses indicate that the richness and diversity of bacterial communities were higher in the three × 'Neva' habitats than in those of fungi. Bacterial and fungal genetic-distance-clustering results were similar; rhizosphere soil clustered in one category, with roots and phloem in another. The diversity and evenness values of the microbial community were: rhizosphere soil > phloem > root system. The bacterial communities in the three habitats were dominated by the Proteobacteria, and fungal communities were dominated by the Ascomycota. The community composition and abundance of each part were quite different; those of 'Neva' were similar among seasons, but community abundance fluctuated. Seasonal fluctuation in the bacterial community was greatest in rhizosphere soil, while that of the fungal community was greatest in phloem. The transgenic lines ECAA1 and ECAB1 had a bacterial and fungal community composition similar to that of the control samples, with no significant differences in community structure or diversity among the lines. The abundances of operational taxonomic units (OTUs) were low, and differed significantly among the lines. These differences did not affect the functioning of the whole specific community. Sampling time and location were the main driving factors of changes in the × 'Neva' microbial community. Transgenic events did not affect the × 'Neva' rhizosphere or endophytic microbial communities. This study provides a reference for the safety evaluation of transgenic plants and the internal colonization mechanism of microorganisms in plants.
探索微生物群落复杂的时空变化及定殖机制，将有助于更好地利用微生物群落服务于农业和生态活动。此外，评估转基因植物对其内生微生物群落的影响对于其商业应用来说是必要的。在本研究中，对不同季节采集的携带c-Cry3A-BADH基因的בNeva’（ECAA1系）、携带基因的בNeva’（ECAB1系）以及非转基因בNeva’的根际土壤、根和韧皮部的微生物群落进行了比较分析。我们的分析表明，在这三种בNeva’生境中，细菌群落的丰富度和多样性高于真菌群落。细菌和真菌的遗传距离聚类结果相似；根际土壤聚为一类，根和韧皮部聚为另一类。微生物群落的多样性和均匀度值为：根际土壤＞韧皮部＞根系。这三种生境中的细菌群落以变形菌门为主，真菌群落以子囊菌门为主。各部分的群落组成和丰度差异较大；‘Neva’的在不同季节相似，但群落丰度有波动。细菌群落的季节性波动在根际土壤中最大，而真菌群落的在韧皮部中最大。转基因系ECAA1和ECAB1的细菌和真菌群落组成与对照样品相似，各系之间的群落结构或多样性无显著差异。可操作分类单元（OTU）的丰度较低，且各系之间差异显著。这些差异并未影响整个特定群落的功能。采样时间和地点是בNeva’微生物群落变化的主要驱动因素。转基因事件并未影响בNeva’的根际或内生微生物群落。本研究为转基因植物的安全性评价及微生物在植物体内的定殖机制提供了参考。
