Zhang Ting, Li Ya-Nan, Li Xue, Gu Wangliu, Moeketsi Emily Kolojane, Zhou Ruiwen, Zheng Xiaobo, Zhang Zhengguang, Zhang Haifeng
Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing, China.
Front Plant Sci. 2022 Jan 26;12:811041. doi: 10.3389/fpls.2021.811041. eCollection 2021.
Fatty acid metabolism is important for the maintenance of fatty acid homeostasis. Free fatty acids, which are toxic in excess, are activated by esterification with coenzyme A (CoA) and then subjected to β-oxidization. Fatty acid β-oxidation-related genes play critical roles in the development and virulence of several phytopathogens. In this study, we identified and characterized a peroxisomal-CoA synthetase in the rice blast fungus , , which is a homolog of in budding yeast. was highly expressed during the conidial and early infectious stages and was induced under oleate treatment. Targeted deletion of resulted in a significant reduction in growth rate when oleate and olive oil were used as the sole carbon sources. Compared with the wild-type strain Guy11, the Δ mutant exhibited fewer peroxisomes, more lipid droplets, and decreased pathogenicity. The distribution of MoPcs60 varied among developmental stages and was mainly localized to peroxisomes in the hyphae, conidia, and appressoria when treated with oleate. Our results suggest that MoPcs60 is a key peroxisomal-CoA synthetase involved in fatty acid β-oxidation and pathogenicity in rice blast fungi.
脂肪酸代谢对于维持脂肪酸稳态至关重要。游离脂肪酸过量时具有毒性，通过与辅酶A（CoA）酯化而被激活，然后进行β-氧化。脂肪酸β-氧化相关基因在几种植物病原体的发育和毒力中起关键作用。在本研究中，我们在稻瘟病菌中鉴定并表征了一种过氧化物酶体-CoA合成酶，它是芽殖酵母中[具体基因名称未给出]的同源物。[该基因名称未给出]在分生孢子和早期感染阶段高度表达，并在油酸盐处理下被诱导。当以油酸盐和橄榄油作为唯一碳源时，对[该基因名称未给出]进行靶向缺失导致生长速率显著降低。与野生型菌株Guy11相比，Δ突变体表现出过氧化物酶体数量减少、脂滴增多以及致病性降低。在用油酸盐处理时，MoPcs60的分布在不同发育阶段有所不同，主要定位于菌丝、分生孢子和附着胞中的过氧化物酶体。我们的结果表明，MoPcs60是参与稻瘟病菌脂肪酸β-氧化和致病性的关键过氧化物酶体-CoA合成酶。
