Bian Xiaofeng, Kim Ho Soo, Kwak Sang-Soo, Zhang Qian, Liu Shuai, Ma Peiyong, Jia Zhaodong, Xie Yizhi, Zhang Peng, Yu Yang
Institute of Food Crops, Provincial Key Laboratory of Agrobiology, Jiangsu Academy of Agricultural Sciences, Nanjing, China.
Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, South Korea.
Front Plant Sci. 2022 Jan 26;13:820450. doi: 10.3389/fpls.2022.820450. eCollection 2022.
Plant root systems are essential for the uptake of water and nutrients from soil and are positively correlated to yield in many crops including the sweetpotato, (L.) Lam. Here, we isolated and functionally characterized , a novel nuclear-localized gene encoding the AP2/ERF transcription factor, from sweetpotato. was responsive to NaCl, PEG8000, ethylene, and Indole 3-acetic acid treatments. As revealed by electrophoretic mobility shift assay and dual luciferase assay, IbRAP2.4 could bind to both DRE and GCC-box elements and acted as a transcription activator. overexpression significantly promoted lateral root formation and enhanced the drought tolerance in , while it inhibited storage root formation in transgenic sweetpotato by comprehensively upregulating lignin biosynthesis pathway genes. Results suggested that may be a useful potential target for further molecular breeding of high yielding sweetpotato.
植物根系对于从土壤中吸收水分和养分至关重要，并且在包括甘薯（Ipomoea batatas (L.) Lam.）在内的许多作物中与产量呈正相关。在此，我们从甘薯中分离出一个编码AP2/ERF转录因子的新型核定位基因IbRAP2.4，并对其进行了功能表征。IbRAP2.4对NaCl、PEG8000、乙烯和吲哚-3-乙酸处理有响应。电泳迁移率变动分析和双荧光素酶分析表明，IbRAP2.4可以与DRE和GCC-box元件结合并作为转录激活因子发挥作用。IbRAP2.4过表达显著促进了甘薯侧根的形成并增强了其耐旱性，同时通过全面上调木质素生物合成途径基因抑制了转基因甘薯中贮藏根的形成。结果表明，IbRAP2.4可能是进一步进行高产甘薯分子育种的一个有用潜在靶点。
