前列腺素F在胚泡着床中的可能作用。

Possible role of prostaglandin F in blastocyst implantation.

作者信息

Kasamo M, Ishikawa M, Yamashita K, Sengoku K, Shimizu T

出版信息

Prostaglandins. 1986 Feb;31(2):321-36. doi: 10.1016/0090-6980(86)90057-2.

Abstract

The synthesis and release of Prostaglandin F (PGF) by the rabbit blastocyst and endometrium were investigated on Day 6 and Day 7, using radioimmunoassay, autoradiography and conversion experiments. The following results were obtained: The content of PGF in the blastocyst increased significantly (P less than 0.01) from Day 6 to Day 7. The content of PGF in the endometrium was significantly higher (P less than 0.05) on Day 7 implantation sites compared to the other areas. The in vitro synthesis and release of PGF by Day 6 blastocysts sharply increased after one and two hours of culture, respectively. Thereafter both values declined with time. The in vitro synthesis and release of PGF by Day 6 endometria increased continuously with time. 14C-arachidonic acid (14C-AA) was incorporated into Day 6 blastocysts in vitro and converted to PGF2 alpha. These results suggest that both the endometrium and the blastocyst are the sources of the PGs involved in implantation, and that PGF derived from the blastocysts may act as the trigger of implantation.

摘要

采用放射免疫分析法、放射自显影法及转化实验，对家兔胚泡和子宫内膜在第6天和第7天时前列腺素F（PGF）的合成与释放进行了研究。获得了以下结果：从第6天到第7天，胚泡中PGF的含量显著增加（P<0.01）。与其他区域相比，在第7天的着床部位，子宫内膜中PGF的含量显著更高（P<0.05）。培养1小时和2小时后，第6天胚泡PGF的体外合成与释放分别急剧增加。此后，这两个值均随时间下降。第6天子宫内膜PGF的体外合成与释放随时间持续增加。体外培养时，第6天的胚泡将14C-花生四烯酸（14C-AA）摄取并转化为PGF2α。这些结果表明，子宫内膜和胚泡都是参与着床的前列腺素的来源，并且来自胚泡的PGF可能是着床的触发因素。