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热休克蛋白90抑制剂N-乙烯基吡咯烷酮-AUY922对胶质母细胞瘤癌干细胞样细胞的优先放射增敏作用

Preferential radiosensitization to glioblastoma cancer stem cell-like cells by a Hsp90 inhibitor, N-vinylpyrrolidone-AUY922.

作者信息

Tani Toshiaki, Tojo Naomi, Ohnishi Ken

机构信息

Radiological Technology Section, QST Hospital, National Institutes for Quantum and Radiological Science and Technology, Chiba 263-8555, Japan.

Department of Biology, Ibaraki Prefectural University of Health Sciences, Inashiki, Ibaraki 300-0394, Japan.

出版信息

Oncol Lett. 2022 Mar;23(3):102. doi: 10.3892/ol.2022.13222. Epub 2022 Jan 31.

DOI:10.3892/ol.2022.13222
PMID:35154433
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8822487/
Abstract

The present study examined the radiosensitization induced by a heat shock protein 90 inhibitor, N-vinylpyrrolidone (NVP)-AUY922, in CD133-positive cells in a hypoxic area of T98G spheroids. CD133-positive cells that are induced in the hypoxic microenvironment of spheroids have previously been reported to exhibit cancer stem cell-like properties. The present study used CD133-positive cells from a glioblastoma cell line (T98G) as cancer stem cell-like cells. CD133-positive and negative cells were sorted from T98G spheroids using fluorescence-activated cell sorting and used for colony formation assay. Colony formation assay results indicated that NVP-AUY922 enhanced radiosensitivity more strongly in CD133-positive cells compared with CD133-negative cells. This result showed that NVP-AUY922 was a preferential radiosensitization candidate targeting glioblastoma cancer stem cells. The mechanisms underlying radiosensitization by NVP-AUY922 are discussed in relation to the properties of cancer stem cells. Overall, HIF-1α inhibition by NVP-AUY922 may induce higher sensitization of cancer stem cells to radiation.

摘要

本研究检测了热休克蛋白90抑制剂N-乙烯基吡咯烷酮（NVP）-AUY922对T98G球体缺氧区域中CD133阳性细胞的放射增敏作用。先前有报道称，在球体缺氧微环境中诱导产生的CD133阳性细胞具有癌症干细胞样特性。本研究使用来自胶质母细胞瘤细胞系（T98G）的CD133阳性细胞作为癌症干细胞样细胞。利用荧光激活细胞分选技术从T98G球体中分选CD133阳性和阴性细胞，并用于集落形成试验。集落形成试验结果表明，与CD133阴性细胞相比，NVP-AUY922在CD133阳性细胞中增强放射敏感性的作用更强。该结果表明，NVP-AUY922是一种靶向胶质母细胞瘤癌症干细胞的优先放射增敏候选药物。结合癌症干细胞的特性讨论了NVP-AUY922放射增敏的潜在机制。总体而言，NVP-AUY922对HIF-1α的抑制作用可能会使癌症干细胞对辐射产生更高的敏感性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7747/8822487/f8e704b459db/ol-23-03-13222-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7747/8822487/83691d53cf1a/ol-23-03-13222-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7747/8822487/59a0f42941bc/ol-23-03-13222-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7747/8822487/07c697101faa/ol-23-03-13222-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7747/8822487/f8e704b459db/ol-23-03-13222-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7747/8822487/83691d53cf1a/ol-23-03-13222-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7747/8822487/59a0f42941bc/ol-23-03-13222-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7747/8822487/07c697101faa/ol-23-03-13222-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7747/8822487/f8e704b459db/ol-23-03-13222-g03.jpg

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本文引用的文献

1
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Biochem Biophys Res Commun. 2021 Mar 26;546:150-154. doi: 10.1016/j.bbrc.2021.02.009. Epub 2021 Feb 11.
2
Clinical Evaluation and Biomarker Profiling of Hsp90 Inhibitors.Hsp90抑制剂的临床评估与生物标志物分析
Methods Mol Biol. 2018;1709:423-441. doi: 10.1007/978-1-4939-7477-1_29.
3
Mechanisms of Resistance to Hsp90 Inhibitor Drugs: A Complex Mosaic Emerges.Hsp90抑制剂药物的耐药机制：一幅复杂的拼图逐渐显现。
靶向恶性脑肿瘤中的热休克蛋白：从基础研究到临床试验
Cancers (Basel). 2022 Nov 4;14(21):5435. doi: 10.3390/cancers14215435.
4
Small Molecules and Immunotherapy Agents for Enhancing Radiotherapy in Glioblastoma.用于增强胶质母细胞瘤放疗效果的小分子和免疫治疗药物
Biomedicines. 2022 Jul 21;10(7):1763. doi: 10.3390/biomedicines10071763.
Pharmaceuticals (Basel). 2011 Oct 25;4(11):1400-1422. doi: 10.3390/ph4111400.
4
Hsp90: A Global Regulator of the Genotype-to-Phenotype Map in Cancers.热休克蛋白90：癌症中基因型到表型图谱的全局调节因子。
Adv Cancer Res. 2016;129:225-47. doi: 10.1016/bs.acr.2015.11.001. Epub 2015 Nov 29.
5
Cancer stem cells in glioblastoma.胶质母细胞瘤中的癌症干细胞。
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6
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8
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9
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Cancer Biol Ther. 2013 Apr;14(4):347-56. doi: 10.4161/cbt.23626. Epub 2013 Jan 28.
10
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PLoS One. 2012;7(4):e35436. doi: 10.1371/journal.pone.0035436. Epub 2012 Apr 16.