用于无标记细胞和纳米颗粒定量动态成像的光学计算相显微镜。
Optically computed phase microscopy for quantitative dynamic imaging of label-free cells and nanoparticles.
作者信息
Liu Xuan, Wan Zhaoxiong, Zhang Yuanwei, Liu Yuwei
机构信息
Department of Electrical and Computer Engineering, New Jersey Institute of Technology, University Heights, Newark, New Jersey 07102, USA.
Department of Chemistry and Environmental Science, New Jersey Institute of Technology, University Heights, Newark, New Jersey 07102, USA.
出版信息
Biomed Opt Express. 2021 Dec 24;13(1):514-524. doi: 10.1364/BOE.449034. eCollection 2022 Jan 1.
Numerous drug delivery systems based on nanoparticles have been developed, such as those used in BioNTech/Pfizer's and Moderna's Covid vaccines. Knowledge on mechanical interactions between cells and nanoparticles is critical to advance the efficiency and safety of these drug delivery systems. To quantitatively track the motion of cell (transparent) and nanoparticles (nontransparent) with nanometer displacement sensitivity, we investigate a novel imaging technology, optically computed phase microscopy (OCPM) that processes 3D spatial-spectral data through optical computation. We demonstrate that OCPM has the capability to image the motion of cells and magnetic nanoparticles that are mechanically excited by an external magnetic field, quantitatively and in the en face plane.
已经开发了许多基于纳米颗粒的药物递送系统，例如用于BioNTech/辉瑞和莫德纳新冠疫苗中的那些系统。了解细胞与纳米颗粒之间的机械相互作用对于提高这些药物递送系统的效率和安全性至关重要。为了以纳米级位移灵敏度定量跟踪细胞（透明）和纳米颗粒（不透明）的运动，我们研究了一种新颖的成像技术——光学计算相显微镜（OCPM），它通过光学计算来处理三维空间光谱数据。我们证明，OCPM有能力在正平面上定量成像细胞和被外部磁场机械激发的磁性纳米颗粒的运动。
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