Tietze Lisa, Mangold Antonia, Hoff Maria W, Lale Rahmi
Department of Biotechnology and Food Science, Faculty of Natural Sciences, Norwegian University of Science and Technology, Trondheim, Norway.
Front Bioeng Biotechnol. 2022 Jan 27;10:826142. doi: 10.3389/fbioe.2022.826142. eCollection 2022.
has recently gained attention as a novel fast-growing bacterium in synthetic biology applications. Currently, a limited set of genetic elements optimised for are used in due to the lack of DNA parts characterised in this novel host. In this study, we report the identification and cross-characterisation of artificial promoters and 5' untranslated regions (artificial regulatory sequence, ARES) that lead to production of fluorescent proteins with a wide-range of expression levels. We identify and cross-characterise 52 constructs in and . Furthermore, we report the DNA sequence and motif analysis of the ARESs using various algorithms. With this study, we expand the pool of characterised genetic DNA parts that can be used for different biotechnological applications using as a host microorganism.
作为合成生物学应用中的一种新型快速生长细菌,近来受到了关注。目前,由于在这种新型宿主中缺乏经表征的DNA元件,因此在[具体应用]中使用的针对[具体对象]优化的遗传元件有限。在本研究中,我们报告了人工启动子和5'非翻译区(人工调控序列,ARES)的鉴定及交叉表征,这些人工启动子和5'非翻译区可导致产生具有广泛表达水平的荧光蛋白。我们在[具体实验对象1]和[具体实验对象2]中鉴定并交叉表征了52个构建体。此外,我们使用各种算法报告了ARES的DNA序列和基序分析。通过这项研究,我们扩大了可用于以[具体宿主微生物]作为宿主微生物的不同生物技术应用的经表征的遗传DNA元件库。