as a pET-Compatible Expression Host Complementary to .

Efficient and novel recombinant protein expression systems can further reduce the production cost of enzymes. is the fastest growing free-living bacterium with a doubling time of less than 10 min, which makes it highly attractive as a protein expression host. Here, 196 pET plasmids with different genes of interest (GOIs) were electroporated into the strain VnDX, which carries an integrated T7 RNA polymerase expression cassette. As a result, 65 and 75% of the tested GOIs obtained soluble expression in and , respectively, 20 GOIs of which showed better expression in the former. Furthermore, we have adapted a consensus "what to try first" protocol for based on Terrific Broth medium. Six sampled GOIs encoding biocatalysts enzymes thus achieved 50-128% higher catalytic efficiency under the optimized expression conditions. Our study demonstrated as a pET-compatible expression host with a spectrum of highly expressed GOIs distinct from and an easy-to-use consensus protocol, solving the problem that some GOIs cannot be expressed well in .