Wang Bo, Wang Shanshan, Ding Manyi, Lu Han, Wu Hao, Li Yao
Institute of Animal Nutrition, College of Animal Science and Technology, Northeast Agricultural University, Harbin, China.
Front Vet Sci. 2022 Jan 27;8:786519. doi: 10.3389/fvets.2021.786519. eCollection 2021.
This study intended to explore the effect and mechanism of different doses of dietary quercetin on calcium and phosphorus metabolism to provide an experimental basis for preventing leg disease in broilers. A total of 480 1-day-old healthy Arbor Acre broilers were randomly allotted into four groups (0, 0.02, 0.04, 0.06%) for 42 days. Compared with control, 0.06% quercetin significantly increased the unit weight and the relative weight of tibia in broilers ( < 0.05). Meanwhile, phosphorus content and bone mineral density (BMD) were significantly increased by 0.06% dietary quercetin supplementation in tibia ( < 0.05). Ash of tibia was significantly increased by 0.04 and 0.06% quercetin in broilers ( < 0.05). In addition, 0.06% quercetin significantly increased the content of serum calcium-binding protein (CB), estradiol (E), osteocalcin (OC), alkaline phosphatase (ALP), and calcitonin (CT) ( < 0.05); 0.04% quercetin significantly increased 1,25-dihydroxyvitamin D (1,25-(OH)D) ( < 0.05) content in serum of broilers. The content of serum parathyroid (PTH) was significantly decreased by 0.02 and 0.06% quercetin ( < 0.05) in broilers. Gene Ontology (GO) functional annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that the Wnt signaling pathway was a key signaling pathway of calcium and phosphorus metabolism in broilers which was significantly regulated by quercetin. The differentially expressed genes (DEGs) from transcriptome sequencing were validated with real-time quantitative PCR (RT-qPCR). In conclusion, 0.06% dietary quercetin supplementation improved calcium and phosphorus metabolism by regulating the Wnt signaling pathway in broilers.
本研究旨在探讨不同剂量的日粮槲皮素对钙磷代谢的影响及机制，为预防肉鸡腿部疾病提供实验依据。将480只1日龄健康艾拔益加肉鸡随机分为四组（0%、0.02%、0.04%、0.06%），为期42天。与对照组相比，0.06%槲皮素显著增加了肉鸡的单位体重和胫骨相对重量（P<0.05）。同时，日粮添加0.06%槲皮素显著提高了胫骨中的磷含量和骨密度（BMD）（P<0.05）。0.04%和0.06%槲皮素显著增加了肉鸡胫骨的灰分（P<0.05）。此外，0.06%槲皮素显著提高了血清钙结合蛋白（CB）、雌二醇（E）、骨钙素（OC）、碱性磷酸酶（ALP）和降钙素（CT）的含量（P<0.05）；0.04%槲皮素显著提高了肉鸡血清中1,25-二羟基维生素D（1,25-(OH)D）的含量（P<0.05）。0.02%和0.06%槲皮素显著降低了肉鸡血清甲状旁腺素（PTH）的含量（P<0.05）。基因本体（GO）功能注释和京都基因与基因组百科全书（KEGG）富集分析表明，Wnt信号通路是肉鸡钙磷代谢的关键信号通路，受槲皮素显著调控。通过实时定量PCR（RT-qPCR）验证了转录组测序中的差异表达基因（DEG）。综上所述，日粮添加0.06%槲皮素通过调节肉鸡的Wnt信号通路改善了钙磷代谢。
