Division of Oral and Maxillofacial Surgery, Department of Dentistry, Korea University Ansan Hospital, Ansan 15355, Republic of Korea.
Department of Periodontics, College of Medicine, The Catholic University of Korea, Seoul 06591, Republic of Korea.
Biomed Res Int. 2022 Feb 4;2022:5884974. doi: 10.1155/2022/5884974. eCollection 2022.
This study evaluated the effects of multiple mouthwashes on the cellular viability or the morphology of preosteoblasts. Mouse calvarial osteoblast-like cells were cultured and treated with mouthwashes of (1) benzydamine hydrochloride; (2) cetylpyridinium chloride and benzalkonium chloride; (3) methyl salicylate, menthol, eucalyptol, and thymol; and (4) sodium fluoride, xylitol, and chitosan. The treatment times were 30 seconds, 90 seconds, and 270 seconds. Cell morphology was evaluated with a microscope, and the viability of the treated cells was analyzed quantitatively using a commercially available kit. The untreated control group exhibited well-stretched fibroblast-like morphology. Treatment with mouthwash resulted in morphological changes in all groups. Treatment with sodium fluoride resulted in more noticeable changes. Treatment with mouthwash for 30 seconds produced a significant decrease in cell viability. An increase in time to 90 and 270 seconds did not produce additional noticeable changes. To conclude, commercially available mouthwashes created changes in cell morphology and decreased the cell viability of osteoblast-like cells irrespective of ingredients and treatment time.
本研究评估了多种漱口水对前成骨细胞的细胞活力或形态的影响。将鼠颅骨成骨样细胞进行培养，并分别用（1）盐酸苯扎氯铵；（2）西吡氯铵和苯扎氯铵；（3）水杨酸甲酯、薄荷醇、桉油醇和百里香酚；和（4）氟化钠、木糖醇和壳聚糖的漱口水进行处理。处理时间分别为 30 秒、90 秒和 270 秒。用显微镜评估细胞形态，并用市售试剂盒定量分析处理后细胞的活力。未经处理的对照组表现出伸展良好的成纤维细胞样形态。所有组经漱口水处理后均发生形态变化。用氟化钠处理后变化更明显。用漱口水处理 30 秒会导致细胞活力显著下降。处理时间延长至 90 秒和 270 秒不会产生其他明显变化。总之，市售漱口水会改变细胞形态并降低成骨样细胞的细胞活力，而与成分和处理时间无关。
