Department of Oral Surgery, University Clinic of Düsseldorf, 40225 Düsseldorf, Germany.
Department of Neurosciences, University of Padua, 35128 Padua, Italy.
Int J Mol Sci. 2021 Sep 15;22(18):9986. doi: 10.3390/ijms22189986.
Several decontamination methods for removing biofilm from implant surfaces during surgical peri-implantitis treatment have been reported, including the intraoperative usage of chlorhexidine (CHX)-based antiseptics. There is a lack of information on possible adverse effects on bone healing. The study aimed to examine the impact of three CHX-based mouthwashes on osteoblast-like cells (SaOS-2) in vitro. Cells were cultured for three days in 96-well binding plates. Each well was randomly treated for either 30, 60 or 120 s with 0.05% CHX combined with 0.05% cetylpyridinium chloride (CPC), 0.1% CHX, 0.2% CHX or sterile saline (NaCl) as control. Cell viability, cytotoxicity and apoptosis were assessed at day 0, 3 and 6. Cell viability resulted in being higher in the control group at all time points. At day 0, the CHX 0.2 group showed significantly higher cytotoxicity values compared to CHX 0.1 (30 s), CHX + CPC (30 s, 60 s and 120 s) and control (60 s and 120 s), while no significant differences were identified between CHX + CPC and both CHX 0.1 and NaCl groups. All test mouthwashes were found to induce apoptosis to a lower extent compared to control. Results indicate that 0.2% CHX presented the highest cytotoxic effect. Therefore, its intraoperative use should be carefully considered.
已有多种针对种植体周围炎手术治疗时去除种植体表面生物膜的消毒方法的报道，包括术中使用含洗必泰（CHX）的防腐剂。但对于这些方法可能对骨愈合产生的不良影响，目前信息有限。本研究旨在体外检测三种不同含 CHX 漱口液对成骨样细胞（SaOS-2）的影响。细胞在 96 孔结合板中培养 3 天。每个孔随机用 0.05%CHX 联合 0.05%西吡氯铵（CPC）、0.1%CHX、0.2%CHX 或无菌生理盐水（NaCl）处理 30、60 或 120 秒，后者作为对照。分别在第 0、3 和 6 天评估细胞活力、细胞毒性和细胞凋亡。所有时间点细胞活力均在对照组中更高。第 0 天，CHX 0.2 组与 CHX 0.1（30 秒）、CHX+CPC（30、60 和 120 秒）和对照组（60 和 120 秒）相比，细胞毒性值明显更高，而 CHX+CPC 与 CHX 0.1 和 NaCl 组之间无显著差异。与对照组相比，所有测试漱口液诱导的细胞凋亡程度均较低。结果表明，0.2%CHX 具有最高的细胞毒性作用。因此，术中使用应谨慎考虑。
