State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, National Clinical Research Center for Oral Diseases, Sichuan University, Chengdu, China.
Department of Operative Dentistry and Endodontics, West China School of Stomatology, Sichuan University, Chengdu, China.
Drug Deliv. 2022 Dec;29(1):561-573. doi: 10.1080/10717544.2022.2037788.
Dental caries is a biofilm-related preventable infectious disease caused by interactions between the oral bacteria and the host's dietary sugars. As the microenvironments in cariogenic biofilms are often acidic, pH-sensitive drug delivery systems have become innovative materials for dental caries prevention in recent years. In the present study, poly(DMAEMA-co-HEMA) was used as a pH-sensitive carrier to synthesize a chlorhexidine (CHX)-loaded nanomaterial (p(DH)@CHX). , p(DH)@CHX exhibited good pH sensitivity and a sustained and high CHX release rate in the acidic environment. It also exhibited lower cytotoxicity against human oral keratinocytes (HOKs) compared to free CHX. Besides, compared with free CHX, p(DH)@CHX showed the same antibacterial effects on biofilms. In addition, it had no effect on eradicating healthy saliva-derived biofilm, while free CHX exhibited an inhibitory effect. Furthermore, the 16s rDNA sequencing results showed that p(DH)@CHX had the potential to alter oral microbiota composition and possibly reduce caries risk. In conclusion, the present study presents an alternative option to design an intelligent material to prevent and treat dental caries.
龋齿是一种由口腔细菌与宿主膳食糖相互作用引起的生物膜相关可预防感染性疾病。由于致龋生物膜中的微环境通常呈酸性，因此 pH 敏感型药物输送系统近年来已成为预防龋齿的创新材料。在本研究中，聚（DMAEMA-co-HEMA）被用作 pH 敏感载体来合成载有氯己定（CHX）的纳米材料（p(DH)@CHX）。p(DH)@CHX 在酸性环境中表现出良好的 pH 敏感性和持续且高的 CHX 释放率。与游离 CHX 相比，它对人口腔角质细胞（HOK）的细胞毒性也较低。此外，与游离 CHX 相比，p(DH)@CHX 对生物膜具有相同的抗菌作用。此外，它对清除健康唾液来源的生物膜没有影响，而游离 CHX 则表现出抑制作用。此外，16s rDNA 测序结果表明，p(DH)@CHX 具有改变口腔微生物群落组成并可能降低龋齿风险的潜力。总之，本研究提出了一种替代方案，设计一种智能材料来预防和治疗龋齿。
