Scientific Education Section, Jinan Stomatological Hospital, Jinan, China.
Department of Endodontics, Jinan Stomatological Hospital, Gao Xin Branch, Jinan, China.
Bioengineered. 2022 Feb;13(2):4226-4234. doi: 10.1080/21655979.2022.2027174.
This study aimed to explore the role of circular-CDC like kinase 1 (circ-CLK1) in the pathogenesis of oral squamous cell carcinoma (OSCC). Circ-CLK1 expression levels were detected via reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The effects of circ-CLK1 knockdown on the viability and apoptosis of OSCC cells were determined using the cell counting kit-8 (CCK-8) assay, EdU staining, flow cytometry, and Western blotting. StarBase and TargetScan were used to predict targeting relationships, which were then confirmed by the dual luciferase reporter assay and RNA pull-down assay. We found that the expression of circ-CLK1 was significantly higher in OSCC patients and cell lines. Inhibition of circ-CLK1 reduced the viability and proliferation of OSCC cells while enhancing their apoptosis. However, inhibiting miR-18b-5p or overexpression of Y-box protein 2 (YBX2) can reverse the effect of circ-CLK1 knockdown on OSCC cells. Therefore, circ-CLK1 inhibited the apoptosis of OSCC cells through the miR-18b-5p/YBX2 axis, and these findings suggest that circ-CLK1 could be a potential therapeutic target for OSCC patients.
本研究旨在探讨环状细胞周期蛋白依赖性激酶 1（circ-CLK1）在口腔鳞状细胞癌（OSCC）发病机制中的作用。通过逆转录定量聚合酶链反应（RT-qPCR）检测 circ-CLK1 的表达水平。使用细胞计数试剂盒-8（CCK-8）测定、EdU 染色、流式细胞术和 Western blot 检测 circ-CLK1 敲低对 OSCC 细胞活力和凋亡的影响。StarBase 和 TargetScan 用于预测靶向关系，然后通过双荧光素酶报告基因测定和 RNA 下拉测定进行验证。我们发现，circ-CLK1 在 OSCC 患者和细胞系中的表达明显升高。抑制 circ-CLK1 降低了 OSCC 细胞的活力和增殖，同时增强了它们的凋亡。然而，抑制 miR-18b-5p 或过表达 Y 盒蛋白 2（YBX2）可以逆转 circ-CLK1 敲低对 OSCC 细胞的影响。因此，circ-CLK1 通过 miR-18b-5p/YBX2 轴抑制 OSCC 细胞的凋亡，这些发现表明 circ-CLK1 可能是 OSCC 患者的潜在治疗靶点。
