Department of General Emergency, The Affiliated Stomatological Hospital of Nanchang University, Nanchang, Jiangxi Province, China.
The Key Laboratory of Oral Biomedicine, Nanchang, Jiangxi Province, China.
J Clin Lab Anal. 2022 Jul;36(7):e24486. doi: 10.1002/jcla.24486. Epub 2022 May 16.
Oral squamous cell carcinoma (OSCC) is one of the most common oral malignant tumors. circ_0004872 can inhibit the progression of gastric cancer, but its effect on the growth and metastasis of OSCC is still unclear.
qRT-PCR was used to detect the expression levels of circ_0004872 and miR-424-5p in cancer tissues of OSCC patients and adjacent normal tissues, OSCC cell lines, and human normal oral keratinocytes (HOK). CCK-8, cell colony formation, flow cytometry, and transwell assay were used to detect cell proliferation rate, viability, apoptosis rate, and invasion ability. Use glucose/lactic acid kit to assay cell glycolysis ability. The dual-luciferase reporter gene experiment and RIP experiment verified the relationship between circ_0004872 and miR-424-5p. The protein levels were examined by Western blot.
The expression of circ_0004872 was significantly downregulated in OSCC tissues and cells, and the overexpression of circ_0004872 inhibited the proliferation, vitality, invasion, and glycolysis of OSCC cells, and promoted apoptosis. The expression of miR-424-5p was greatly upregulated in OSCC tissues and OSCC cells. circ_0004872 can adsorb miR-424-5p in OSCC cells, and circ_0004872 can reverse the promoting effect of miR-424-5p overexpression on the process of OSCC cells.
circ_0004872 suppresses the proliferation, invasion, and glycolysis of OSCC cells by sponged miR-424-5p, and promotes apoptosis, which can be used as a potential target for early diagnosis and targeted therapy of OSCC.
口腔鳞状细胞癌（OSCC）是最常见的口腔恶性肿瘤之一。circ_0004872 可以抑制胃癌的进展，但它对 OSCC 生长和转移的影响尚不清楚。
qRT-PCR 用于检测 OSCC 患者癌组织及相邻正常组织、OSCC 细胞系和人正常口腔角质细胞（HOK）中 circ_0004872 和 miR-424-5p 的表达水平。CCK-8、细胞集落形成、流式细胞术和 Transwell 检测分别用于检测细胞增殖率、活力、凋亡率和侵袭能力。葡萄糖/乳酸试剂盒用于检测细胞糖酵解能力。双荧光素酶报告基因实验和 RIP 实验验证 circ_0004872 与 miR-424-5p 的关系。Western blot 检测蛋白水平。
circ_0004872 在 OSCC 组织和细胞中表达显著下调，circ_0004872 过表达抑制 OSCC 细胞的增殖、活力、侵袭和糖酵解，促进凋亡。miR-424-5p 在 OSCC 组织和 OSCC 细胞中表达显著上调。circ_0004872 可在 OSCC 细胞中吸附 miR-424-5p，circ_0004872 可逆转 miR-424-5p 过表达对 OSCC 细胞过程的促进作用。
circ_0004872 通过吸附 miR-424-5p 抑制 OSCC 细胞的增殖、侵袭和糖酵解，促进凋亡，可作为 OSCC 早期诊断和靶向治疗的潜在靶点。
