State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, Hunan, 410082, P. R. China.
College of Science, Hunan University of Technology and Business, Changsha, Hunan, 410082, P. R. China.
Chem Asian J. 2022 Mar 14;17(6):e202101414. doi: 10.1002/asia.202101414. Epub 2022 Feb 21.
Owing to the high sensitivity, excellent programmability, and flexible obtainment through in vitro selection, RNA-cleaving DNAzymes have attracted increasing interest in developing DNAzyme-based sensors. In this review, we summarize the recent advances on DNAzyme-based sensing applications. We initially conclude two general strategies to expand the library of DNAzmes, in vitro selection to discover new DNAzymes towards different targets of interest and chemical modifications to endue the existing DNAzymes with new function or properties. We then discuss the recent applications of DNAzyme-based sensors for the detection of a variety of important biomoleucles both in vitro and in vivo. Finally, perspectives on the challenges and future directions in the development of DNAzyme-based sensors are provided.
由于 RNA 切割 DNA 酶具有高灵敏度、优异的可编程性以及可通过体外选择灵活获得，因此它们在开发基于 DNA 酶的传感器方面引起了越来越多的关注。在这篇综述中，我们总结了基于 DNA 酶的传感应用的最新进展。我们最初总结了扩展 DNA 酶文库的两种一般策略，即通过体外选择发现针对不同感兴趣目标的新 DNA 酶，以及通过化学修饰赋予现有 DNA 酶新的功能或特性。然后，我们讨论了基于 DNA 酶的传感器在体外和体内检测各种重要生物分子的最新应用。最后，我们对基于 DNA 酶的传感器的发展所面临的挑战和未来方向提供了一些看法。
