Filippidou Myrto Kyriaki, Kanaris Aris Ioannis, Aslanidis Evangelos, Rapesi Annita, Tsounidi Dimitra, Ntouskas Sotirios, Skotadis Evangelos, Tsekenis George, Tsoukalas Dimitris, Tserepi Angeliki, Chatzandroulis Stavros
Institute of Nanoscience and Nanotechnology, NCSR ''Demokritos'', 15341 Aghia Paraskevi, Greece.
Department of Applied Sciences, National Technical University of Athens, 15780 Zografou, Greece.
Micromachines (Basel). 2023 Aug 13;14(8):1595. doi: 10.3390/mi14081595.
The presence of heavy metal ions in soil, air and water constitutes an important global environmental threat, as these ions accumulate throughout the food chain, contributing to the rise of chronic diseases, including, amongst others, cancer and kidney failure. To date, many efforts have been made for their detection, but there is still a need for the development of sensitive, low-cost, and portable devices able to conduct on-site detection of heavy metal ions. In this work, we combine microfluidic technology and electrochemical sensing in a plastic chip for the selective detection of heavy metal ions utilizing DNAzymes immobilized in between platinum nanoparticles (PtNPs), demonstrating a reliable portable solution for water pollution monitoring. For the realization of the microfluidic-based heavy metal ion detection device, a fast and easy-to-implement fabrication method based on the photolithography of dry photosensitive layers is proposed. As a proof of concept, we demonstrate the detection of Pb ions using the prototype microfluidic device.
土壤、空气和水中重金属离子的存在构成了一项重大的全球环境威胁，因为这些离子会在整个食物链中累积，促使包括癌症和肾衰竭等在内的慢性病发病率上升。迄今为止，人们已经为检测这些离子付出了诸多努力，但仍需要开发能够对重金属离子进行现场检测的灵敏、低成本且便携的设备。在这项工作中，我们将微流控技术和电化学传感结合在一个塑料芯片中，利用固定在铂纳米颗粒（PtNPs）之间的DNA酶对重金属离子进行选择性检测，展示了一种用于水污染监测的可靠便携式解决方案。为了实现基于微流控的重金属离子检测装置，提出了一种基于干式光敏层光刻的快速且易于实施的制造方法。作为概念验证，我们展示了使用原型微流控装置对铅离子的检测。
