Lee Jong Min, Jin Chun-Zhi, Park So Hee, Kang Min-Kyoung, Park Dong-Jin, Kim Chang-Jin
Industrial Biomaterial Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon, 34141, Korea.
Department of Bio-Molecular Science, KRIBB School of Bioscience, Korea University of Science and Technology (UST), 217 Gajeong-ro, Yuseong-gu, Daejeon, Korea.
Curr Microbiol. 2021 May;78(5):2130-2135. doi: 10.1007/s00284-021-02370-7. Epub 2021 Mar 11.
A Gram-positive, aerobic, rod-shaped, non-spore-forming bacterium, designated as BN140041, was isolated from cave soil at Gubyeongsan Mountain, Boeun-gun, Chungbuk province in Republic of Korea. Phylogenetic analysis of the 16S rRNA gene sequence showed that the strain is closely related to Nocardioides silvaticus S-34, N. pelophilus THG-T63, and N. immobilis FLL521 with 97.4%, 97.1%, and 96.8% similarity. The draft genome length was 4.27 Mb containing 424 contigs with a DNA G + C content of 70.5 mol%. The ANI value between strain BN140044 and its closely related species N. silvaticus S-34 was 82.6%. The genome sequence of BN140041 displayed a key enzyme involved in the bioremediation of organic pollutants. The diagnostic diamino acid of peptidoglycan was LL-2,6-diaminopimelic acid. The major respiratory quinone was MK-8(H4), and the major fatty acids (> 5% of the total fatty acids) were iso-C (55.3%), Cω9c (7.7%) and iso-C (5.7%). The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, and phosphatidylinositol. The results of genotypical, physiological, and biochemical characterization allow the phenotypic differentiation of strain BN140041 from related the Nocardioides strains. Therefore, strain BN140041 represents a novel species of the genus Nocardioides, for which we propose the name Nocardioides antri sp. nov. The type strain is BN140041 (= KCTC 49080 = CCTCC AB 2018226).
从韩国忠清北道槐山郡九拜山洞穴土壤中分离出一株革兰氏阳性、需氧、杆状、不产芽孢的细菌，命名为BN140041。16S rRNA基因序列的系统发育分析表明，该菌株与森林诺卡氏菌S-34、嗜泥诺卡氏菌THG-T63和固定诺卡氏菌FLL521密切相关，相似性分别为97.4%、97.1%和96.8%。基因组草图长度为4.27 Mb，包含424个重叠群，DNA G+C含量为70.5 mol%。菌株BN140044与其密切相关物种森林诺卡氏菌S-34之间的ANI值为82.6%。BN140041的基因组序列显示出一种参与有机污染物生物修复的关键酶。肽聚糖的诊断性二氨基酸为LL-2,6-二氨基庚二酸。主要呼吸醌为MK-8(H4)，主要脂肪酸(占总脂肪酸的>5%)为异-C(55.3%)、Cω9c(7.7%)和异-C(5.7%)。极性脂质为双磷脂酰甘油、磷脂酰甘油、磷脂酰胆碱和磷脂酰肌醇。基因型、生理和生化特征的结果使菌株BN140041能够与相关的诺卡氏菌菌株进行表型区分。因此，菌株BN140041代表诺卡氏菌属的一个新物种，我们将其命名为洞穴诺卡氏菌（Nocardioides antri sp. nov.）。模式菌株为BN140041(=KCTC 49080=CCTCC AB 2018226)。
