Department of Biotechnology, Pukyong National University, Busan 48513, Republic of Korea.
Department of Food Science and Nutrition, Pukyong National University, Busan 48513, Republic of Korea.
J Microbiol Biotechnol. 2023 May 28;33(5):621-633. doi: 10.4014/jmb.2212.12005. Epub 2023 Feb 6.
We investigated the probiotic characteristics and anti-obesity effect of MGEL20154, a strain that possesses excellent intestinal adhesion and viability. The in vitro properties, , gastrointestinal (GI) resistance, adhesion, and enzyme activity, demonstrated that MGEL20154 is a potential probiotic candidate. Oral administration of MGEL20154 to diet-induced obese C57BL/6J mice for 8 weeks resulted in a feed efficacy decrease by 44.7% compared to that of the high-fat diet (HFD) group. The reduction rate of weight gain was about 48.5% in the HFD+MGEL20154 group compared to that of the HFD group after 8 weeks, and the epididymal fat pad was also reduced in size by 25.2%. In addition, the upregulation of the , , and , and downregulation of the and genes in Caco-2 cells by MGEL20154 were observed. Therefore, we propose that the anti-obesity effect of the strain is exerted by inhibiting carbohydrate absorption and regulating gene expression in the intestine.
我们研究了 MGEL20154 的益生菌特性和抗肥胖作用，该菌株具有良好的肠道黏附性和存活率。体外特性、胃肠道（GI）抗性、黏附性和酶活性表明，MGEL20154 是一种有潜力的益生菌候选菌株。将 MGEL20154 口服给予饮食诱导肥胖的 C57BL/6J 小鼠 8 周，与高脂肪饮食（HFD）组相比，饲料效率降低了 44.7%。与 HFD 组相比，HFD+MGEL20154 组在 8 周后体重增加率降低了约 48.5%，附睾脂肪垫也减少了 25.2%。此外，MGEL20154 还能上调 Caco-2 细胞中的、、和基因，下调、和基因的表达。因此，我们提出该菌株的抗肥胖作用是通过抑制碳水化合物吸收和调节肠道基因表达来实现的。
