Haines D M
Vet Immunol Immunopathol. 1986 Mar;11(3):225-33. doi: 10.1016/0165-2427(86)90003-6.
Serum samples were obtained from 48 dogs with recently diagnosed untreated diabetes mellitus. Serums were tested for cytoplasmic autoantibodies to normal canine pancreatic islet antigens by indirect immunofluorescence, peroxidase-anti-peroxidase, and avidin-biotin complex, immunohistochemistry. Autoantibodies were not detectable in any of the samples. Serums were also examined from 20 diabetic dogs maintained on exogenous insulin therapy for periods of one month to five years. Positive reactions were seen in 11 dogs. These positive responses were completely absorbed by preincubation of serums with commercial insulin preparations or with purified pork or beef insulin. Newly diagnosed diabetic dogs do not have readily detectable autoantibodies to islet cytoplasmic antigens. Our previous report (Haines and Penhale, 1985) of islet antibody in diabetic dogs with unknown clinical histories was likely demonstrating antibody to insulin in patients treated with exogenous insulin. Antibodies to insulin were detected in approximately half of the insulin treated dogs tested. These antibodies were induced by commercial beef and pork insulin preparations and were found to be broadly cross-reactive recognizing epitopes on canine, bovine and porcine insulins.
从48只近期诊断为未经治疗的糖尿病的犬只中获取血清样本。通过间接免疫荧光、过氧化物酶-抗过氧化物酶和抗生物素蛋白-生物素复合物免疫组织化学方法检测血清中针对正常犬胰岛抗原的细胞质自身抗体。在任何样本中均未检测到自身抗体。还对20只接受外源性胰岛素治疗1个月至5年的糖尿病犬的血清进行了检测。11只犬出现阳性反应。这些阳性反应在血清与商业胰岛素制剂或纯化的猪胰岛素或牛胰岛素预孵育后完全被吸收。新诊断的糖尿病犬没有易于检测到的针对胰岛细胞质抗原的自身抗体。我们之前关于临床病史不明的糖尿病犬胰岛抗体的报告（海恩斯和彭黑尔，1985年）可能显示的是接受外源性胰岛素治疗患者体内的胰岛素抗体。在大约一半接受检测的胰岛素治疗犬中检测到胰岛素抗体。这些抗体由商业牛肉和猪肉胰岛素制剂诱导产生，并且发现具有广泛的交叉反应性，能够识别犬、牛和猪胰岛素上的表位。
