School of Life Science, East China Normal University, Shanghai, 200241, China.
School of Life and Environmental Sciences, Hangzhou Normal University, 310018, Hangzhou, Zhejiang, China.
Environ Pollut. 2022 May 1;300:118989. doi: 10.1016/j.envpol.2022.118989. Epub 2022 Feb 11.
Nanoplastic, ubiquitous in aquatic environments, are raising concern worldwide. However, studies on nanoplastic exposure and its effects on ion transport in aquatic organisms are limited. In this study, the juvenile oriental river shrimp, Macrobrachium nipponense, was exposed to five levels of nanoplastic concentrations (0, 5, 10, 20, 40 mg/L) in order to evaluate cell viability, ion content, ion transport, ATPase activity, and related gene expression. The results showed that the apoptosis rate was higher in the high concentration nanoplastic group (40 mg/L) compared to the low concentration nanoplastic group (5 mg/L) and the control group (0 mg/L). The ion content of sodium (Na), potassium (K), chloride (Cl), and calcium (Ca) showed a decreasing trend in gill tissue compared to the control group. The NaK-ATPase, V(H)-ATPase, CaMg-ATPase, and total ATPase activities in the gills of M. nipponense showed a general decrease with the increasement of nanoplastic concentration and time of exposure. When increasing nanoplastic concentration, the expression of ion transport-related genes in the gills of M. nipponense showed first rise then descend trend. As elucidated by the results, high nanoplastic concentrations have negative effect on cell viability, ion content, ion transport ATPase activity, and ion transport-related gene expression in the gills of M. nipponense. This research provides a theoretical foundation for the toxic effects of nanoplastic in aquaculture.
纳米塑料在水生环境中无处不在，引起了全球关注。然而，关于纳米塑料暴露及其对水生生物离子转运影响的研究还很有限。在这项研究中，使用五种不同浓度的纳米塑料（0、5、10、20、40mg/L）对幼年日本沼虾（Macrobrachium nipponense）进行暴露实验，以评估细胞活力、离子含量、离子转运、ATP 酶活性和相关基因表达。结果表明，高浓度纳米塑料组（40mg/L）的细胞凋亡率高于低浓度纳米塑料组（5mg/L）和对照组（0mg/L）。与对照组相比，虾鳃组织中的钠离子（Na）、钾离子（K）、氯离子（Cl）和钙离子（Ca）含量呈下降趋势。虾鳃中的 NaK-ATP 酶、V(H)-ATP 酶、CaMg-ATP 酶和总 ATP 酶活性随着纳米塑料浓度和暴露时间的增加呈普遍下降趋势。随着纳米塑料浓度的增加，虾鳃中离子转运相关基因的表达呈先上升后下降的趋势。研究结果表明，高浓度纳米塑料对日本沼虾鳃细胞活力、离子含量、离子转运、ATP 酶活性和离子转运相关基因表达有负面影响。本研究为纳米塑料在水产养殖中的毒性作用提供了理论基础。
