Jiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang, 222005, China; Marine Resource Development Institute of Jiangsu (Lianyungang), Lianyungang, 222005, China; Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Jiangsu Ocean University, Lianyungang, 222005, China; The Jiangsu Provincial Infrastructure for Conservation and Utilization of Agricultural Germplasm, Nanjing, 210014, China.
Hainan Provincial Key Laboratory of Tropical Maricultural Technologies, Hainan Academy of Ocean and Fisheries Sciences, Haikou, 571126, China.
Fish Shellfish Immunol. 2022 Mar;122:206-214. doi: 10.1016/j.fsi.2022.02.016. Epub 2022 Feb 11.
For commercial aquatic animals, hypoxia phenomenon often occurs in live transport and aquaculture. In previous studies, much interest has been focused on antioxidant enzyme activities and could not present the complexities. The multifaceted responses, especially considering physiological indexes, histological structure, cell apoptosis, and immune pathways, are still unknown. In this study, we investigated the comprehensive hypoxic responses of Marsupenaeus japonicus. The results showed that the physiological indexes showed time-dependent changes upon hypoxia stress. Hypoxia stress led to significant tissue damage and cell apoptosis in the gill and hepatopancreas. Compared with the control group, the apoptosis index (AI) of the 12 h hypoxic treatment increased significantly (p < 0.05) in the gills and hepatopancreas. Comparative transcriptome analysis identified 900 and 1400 differentially expressed genes (DEGs) in the gill and hepatopancreas, respectively. Several DEGs were related to the lysosome, glycolysis/gluconeogenesis, citrate cycle, and apoptosis, and seven of them were validated using quantitative real-time PCR. This study provided valuable clues to understanding the mechanisms underlying the hypoxic responses of M. japonicus.
对于商业水产动物来说，缺氧现象在活体运输和水产养殖中经常发生。在以前的研究中，人们对抗氧化酶活性很感兴趣，但未能呈现出复杂性。多方面的反应，特别是考虑到生理指标、组织学结构、细胞凋亡和免疫途径，仍然未知。在这项研究中，我们研究了日本沼虾的综合缺氧反应。结果表明，生理指标在缺氧应激下表现出时间依赖性变化。缺氧应激导致鳃和肝胰腺组织损伤和细胞凋亡。与对照组相比，12 h 缺氧处理组的鳃和肝胰腺凋亡指数（AI）显著增加（p<0.05）。比较转录组分析分别在鳃和肝胰腺中鉴定出 900 和 1400 个差异表达基因（DEGs）。其中一些 DEGs 与溶酶体、糖酵解/糖异生、柠檬酸循环和细胞凋亡有关，其中 7 个基因使用定量实时 PCR 进行了验证。本研究为了解日本沼虾缺氧反应的机制提供了有价值的线索。
