Scambi Ilaria, Peroni Daniele, Nodari Alice, Merigo Flavia, Benati Donatella, Boschi Federico, Mannucci Silvia, Frontini Andrea, Visonà Silvia, Sbarbati Andrea, Krampera Mauro, Galiè Mirco
Department Neuroscience, Biomedicine and Movement, Sec. Anatomy and Histology, Universitity of Verona, Verona, Italy.
Laboratory of Translational Genomics, Centre for Integrative Biology, University of Trento, Trento, Italy.
Eur J Cell Biol. 2022 Apr;101(2):151206. doi: 10.1016/j.ejcb.2022.151206. Epub 2022 Feb 8.
Multipotent stem cells persist within the stromal vascular fraction (SVF) of adipose tissue during adulthood. These cells, commonly referred to as adipose-derived stromal cells (ASC), have been extensively investigated over the past years as a promising therapeutic tool based on their regenerative and immunomodulatory properties. However, how ASC might mirror the age-related alteration of the fat they reside in remains unclear. Herein, we show that inguinal adipose tissue in mice turns from brown/beige- to white-like with age and resident ASC readily mirror these changes both at mRNA and microRNA transcriptional level. Mechanistically, our data suggest that these brown/age-related changes in ASC transcription rely on changes in the activity of E2F1 and NFkB transcription factors.
多能干细胞在成年期持续存在于脂肪组织的基质血管部分（SVF）中。这些细胞通常被称为脂肪来源的基质细胞（ASC），在过去几年中，基于其再生和免疫调节特性，作为一种有前景的治疗工具受到了广泛研究。然而，ASC如何反映它们所驻留脂肪的与年龄相关的变化仍不清楚。在此，我们表明，小鼠腹股沟脂肪组织随年龄增长从棕色/米色转变为类白色，驻留的ASC在mRNA和微小RNA转录水平上很容易反映这些变化。从机制上讲，我们的数据表明，ASC转录中这些与棕色/年龄相关的变化依赖于E2F1和NFkB转录因子活性的变化。
