通过人多能干细胞和普通狨猴比较转录组分析鉴定同源重组增强因子。
Homologous Recombination-Enhancing Factors Identified by Comparative Transcriptomic Analyses of Pluripotent Stem Cell of Human and Common Marmoset.
机构信息
Department of Physiology, Keio University School of Medicine, Tokyo 160-8582, Japan.
Laboratory for Marmoset Neural Architecture, RIKEN Center for Brain Science, Saitama 351-0198, Japan.
出版信息
Cells. 2022 Jan 21;11(3):360. doi: 10.3390/cells11030360.
A previous study assessing the efficiency of the genome editing technology CRISPR-Cas9 for knock-in gene targeting in common marmoset (marmoset; Callithrix jacchus) embryonic stem cells (ESCs) unexpectedly identified innately enhanced homologous recombination activity in marmoset ESCs. Here, we compared gene expression in marmoset and human pluripotent stem cells using transcriptomic and quantitative PCR analyses and found that five HR-related genes (BRCA1, BRCA2, RAD51C, RAD51D, and RAD51) were upregulated in marmoset cells. A total of four of these upregulated genes enhanced HR efficiency with CRISPR-Cas9 in human pluripotent stem cells. Thus, the present study provides a novel insight into species-specific mechanisms for the choice of DNA repair pathways.
先前有一项研究评估了基因组编辑技术 CRISPR-Cas9 在对普通狨猴（狨猴；Callithrix jacchus）胚胎干细胞（ESCs）进行基因敲入靶向时的效率，该研究出人意料地发现狨猴 ESCs 中存在固有增强的同源重组活性。在这里，我们使用转录组学和定量 PCR 分析比较了狨猴和人类多能干细胞中的基因表达，发现五个与 HR 相关的基因（BRCA1、BRCA2、RAD51C、RAD51D 和 RAD51）在狨猴细胞中上调。其中四个上调基因增强了 CRISPR-Cas9 在人类多能干细胞中的 HR 效率。因此，本研究为选择 DNA 修复途径的物种特异性机制提供了新的见解。
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