Center for Biological Diversity Conservation in Powsin-Polish Academy of Sciences Botanical Garden, Prawdziwka 2, 02-973 Warsaw, Poland.
Department of Ornamental Plants and Garden Art, University of Agriculture in Krakow, 29 Listopada 54, 31-425 Kraków, Poland.
Cells. 2022 Jan 30;11(3):486. doi: 10.3390/cells11030486.
The tree ferns are an important component of tropical forests. In view of this, the enhancement of in vitro production of these plants is needed. Thus, the effect of different light-emitting diodes (LEDs) as well as control fluorescent lamps (Fl) and a 3-week-long period of darkness at the beginning of in vitro culture on micropropagation of the tree fern Sternb. was analysed. Moreover, the photosynthetic pigment content and secondary metabolite profiles were estimated. The period of darkness contributed to a high production of somatic embryo-derived sporophytes and a low production of gametophytes. The formation of new sporophytes was stimulated by RBY (35% red, 15% blue, and 50% yellow) and B (100% blue) lights when the stipe explants or whole young sporophytes were used in the culture, respectively. The elongation of the roots and leaves was stimulated by RBfR light (35% red, 15% blue, and 50% far red), while root production increased under RBY light. The RB (70% red and 30% blue) and B lights stimulated the accumulation of chlorophyll better than Fl light. The most abundant metabolite found in the plant extracts was -5--caffeoylquinic acid (1.013 µg/mg of dry weight). The extract obtained from plants growing in a greenhouse had the best antioxidant activity.
树蕨是热带森林的重要组成部分。鉴于此，需要提高这些植物的体外生产。因此，分析了不同发光二极管（LED）以及对照荧光灯（Fl）和在体外培养开始时长达 3 周的黑暗对树蕨 Sternb 的微繁殖的影响。此外，还估计了光合作用色素含量和次生代谢物谱。黑暗期有助于大量产生体细胞胚衍生的孢子体，而配子体的产量较低。当使用茎段外植体或整个幼孢子体进行培养时，RBY（35％红，15％蓝和 50％黄）和 B（100％蓝）光刺激新孢子体的形成。RBfR 光（35％红，15％蓝和 50％远红）刺激根和叶的伸长，而 RBY 光下根的产量增加。RB（70％红和 30％蓝）和 B 光比 Fl 光更能刺激叶绿素的积累。在植物提取物中发现的最丰富的代谢物是-5--咖啡酰奎宁酸（1.013 µg/mg 干重）。在温室中生长的植物提取物具有最佳的抗氧化活性。
