Horie N, Yokozeki H, Sato K
Am J Physiol. 1986 Apr;250(4 Pt 2):R691-8. doi: 10.1152/ajpregu.1986.250.4.R691.
Thermally induced human eccrine sweat was collected both by simple scraping (SS) and by a polyethylene sweat collector that covered the Vaseline-coated skin of the back to minimize epidermal contamination (CS, clean sweat). Using synthetic chromogenic Kabi S-series substrates, we demonstrated a variety of proteases in both SS and CS, the former being higher than the latter to varying extents. The higher enzyme activity in SS is mainly due to epidermal contamination because abundant protease activity was eluted from the nonperspiring stripped and unstripped skin in vivo and from the stripped stratum corneum itself. The electrophoretic separation of sweat protein has shown that CS contains at least 7 and SS 15 gelatinolytic proteinases. Although some CS proteinases could be derived from the sweat duct, the sweat secretory coil itself is responsible for at least two proteinases at 78 and 25 kilodaltons. The identity and function of these enzymes remain to be studied.
通过简单刮擦（SS）和使用覆盖背部涂有凡士林皮肤的聚乙烯汗液收集器来收集热诱导的人体外泌汗腺汗液，以尽量减少表皮污染（CS，清洁汗液）。使用合成显色卡比S系列底物，我们在SS和CS中均证实了多种蛋白酶，前者在不同程度上高于后者。SS中较高的酶活性主要归因于表皮污染，因为在体内从无汗的去皮和未去皮皮肤以及从去皮角质层本身洗脱了大量蛋白酶活性。汗液蛋白的电泳分离表明，CS至少含有7种，而SS含有15种明胶水解蛋白酶。尽管一些CS蛋白酶可能来源于汗腺导管，但汗腺分泌盘本身至少负责两种分子量分别为78和25千道尔顿的蛋白酶。这些酶的特性和功能仍有待研究。
