Stefan-van Staden Raluca-Ioana, Cioates Negut Catalina, Gheorghe Sorin Sebastian, Sfirloaga Paula
Laboratory of Electrochemistry and PATLAB, National Institute of Research for Electrochemistry and Condensed Matter, 202 Splaiul Independentei St., 060021 Bucharest, Romania.
Faculty of Applied Chemistry and Material Science, Politehnica University of Bucharest, 060021 Bucharest, Romania.
Nanomaterials (Basel). 2022 Jan 28;12(3):460. doi: 10.3390/nano12030460.
Two three-dimensional (3D) stochastic microsensors based on immobilization of protoporphyrin IX (PIX) in single-walled carbon nanotubes (SWCNT) and multi-walled carbon nanotubes (MWCNT) decorated with copper (Cu) and gold (Au) nanoparticles were designed and used for the molecular recognition of isocitrate dehydrogenase 1 (IDH1) and isocitrate dehydrogenase 2 (IDH2) in biological samples (brain tumor tissues, whole blood). The linear concentration ranges obtained for the molecular recognition and quantification of IDH1 and IDH2 were: IDH1 (1 × 10-1 × 10 ng mL) and IDH2 (5 × 10 - 5 × 10 ng mL). The limits of quantification obtained using the proposed microsensors were: 10 fg mL for IDH1 and 5 × 10 fg mL for IDH2. The highest sensitivities were obtained for the microsensor based on MWCNT. High recoveries versus enzyme-linked immunosorbent assay (ELISA) standard method were recorded for the assays of IDH1 and IDH2, all values being higher than 99.00%, with relative standard deviations (RSD) lower than 0.10%.
设计了两种基于将原卟啉IX（PIX）固定在装饰有铜（Cu）和金（Au）纳米颗粒的单壁碳纳米管（SWCNT）和多壁碳纳米管（MWCNT）中的三维（3D）随机微传感器，并将其用于生物样品（脑肿瘤组织、全血）中异柠檬酸脱氢酶1（IDH1）和异柠檬酸脱氢酶2（IDH2）的分子识别。IDH1和IDH2分子识别与定量的线性浓度范围为：IDH1（1×10 - 1×10 ng/mL）和IDH2（5×10 - 5×10 ng/mL）。使用所提出的微传感器获得的定量限为：IDH1为10 fg/mL，IDH2为5×10 fg/mL。基于MWCNT的微传感器具有最高灵敏度。IDH1和IDH2测定相对于酶联免疫吸附测定（ELISA）标准方法具有高回收率，所有值均高于99.00%，相对标准偏差（RSD）低于0.10%。
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