Stefan-van Staden Raluca-Ioana, Musat Oana-Raluca, Gheorghe Damaris-Cristina, Ilie-Mihai Ruxandra-Maria, van Staden Jacobus Koos Frederick
Laboratory of Electrochemistry and PATLAB, 202 Splaiul Independentei Str., 060021 Bucharest, Romania.
Faculty of Chemical Engineering and Biotechnologies, University Politehnica of Bucharest, 011061 Bucharest, Romania.
Nanomaterials (Basel). 2022 Sep 8;12(18):3111. doi: 10.3390/nano12183111.
Two microsensors obtained by the physical immobilization of 5,10,15,20-tetraphenyl-21H,23H-porphine (TPP) and 5,10,15,20-tetrakis (pentafluorophenyl chloride)-21H,23H-iron (III) porphyrin (Fe(TPFPP)Cl) in carbon nanopowder decorated with gold nanoparticles (AuNp) were designed, characterized, validated and used for the molecular recognition and simultaneous ultrasensitive determination of CEA, CA15-3 and HER2 in whole blood. High sensitivities were recorded for both microsensors. Low limits of quantification were recorded for all biomarkers: CEA (12.8 pg mL by using Fe(TPFPP)Cl/AuNp, and 190 fg mL by using TPP/AuNp), CA 15-3 (100 fU mL for both microsensors) and HER2 (3.9 fg mL by using Fe(TPFPP)Cl/AuNp, and 35 fg mL by using TPP/AuNp). A very good correlation between the results obtained using the proposed microsensors and ELISA, certified by the Student t-test, proves that the screening test can be used for ultrasensitive assays of the three biomarkers in whole blood.
通过将5,10,15,20-四苯基-21H,23H-卟啉（TPP）和5,10,15,20-四（五氟苯基氯）-21H,23H-铁（III）卟啉（Fe(TPFPP)Cl）物理固定在装饰有金纳米颗粒（AuNp）的碳纳米粉末中制备了两个微传感器，对其进行了表征、验证，并用于全血中癌胚抗原（CEA）、糖类抗原15-3（CA15-3）和人表皮生长因子受体2（HER2）的分子识别和同时超灵敏测定。两个微传感器均记录到高灵敏度。所有生物标志物均记录到低定量限：CEA（使用Fe(TPFPP)Cl/AuNp时为12.8 pg/mL，使用TPP/AuNp时为190 fg/mL）、CA 15-3（两个微传感器均为100 fU/mL）和HER2（使用Fe(TPFPP)Cl/AuNp时为3.9 fg/mL，使用TPP/AuNp时为35 fg/mL）。使用所提出的微传感器获得的结果与酶联免疫吸附测定（ELISA）之间具有非常好的相关性，经学生t检验验证，证明该筛选试验可用于全血中三种生物标志物的超灵敏检测。
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