利用沙鼠和TYI-S-33培养基对人和动物贾第虫属进行脱囊和培养
Excystation and culturing of human and animal Giardia spp. by using gerbils and TYI-S-33 medium.
作者信息
Wallis P M, Wallis H M
出版信息
Appl Environ Microbiol. 1986 Mar;51(3):647-51. doi: 10.1128/aem.51.3.647-651.1986.
Abstract
Mongolian gerbils were used as an animal model to excyst and host Giardia spp. isolated from meadow voles, dogs, beavers, and humans. Both cysts and trophozoites were used to establish infections. Gerbils were infected with Giardia duodenalis from beaver, dog, and human sources, and the trophozoites were extracted and cultured in Diamond TYI-S-33 medium. The use of gentamicin and ampicillin in the medium, coupled with treatment of gerbils with gentamicin before they were sacrificed, permitted the elimination of trophozoite purification techniques before culturing. An extract of whole bovine calf blood, CLEX, was substituted for fetal bovine serum in TYI-S-33 medium and was found to be both adequate and less expensive.
摘要
蒙古沙鼠被用作动物模型，用于使从草甸田鼠、狗、海狸和人类中分离出的贾第虫属囊肿脱囊并作为宿主。囊肿和滋养体均用于建立感染。沙鼠感染了来自海狸、狗和人类源的十二指肠贾第虫，提取滋养体并在Diamond TYI-S-33培养基中培养。培养基中使用庆大霉素和氨苄青霉素，再加上在处死沙鼠之前用庆大霉素对其进行处理，使得在培养前无需采用滋养体纯化技术。在TYI-S-33培养基中，用全牛犊血提取物CLEX替代胎牛血清，结果发现其既足够用又成本更低。
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Excystation and culturing of human and animal Giardia spp. by using gerbils and TYI-S-33 medium.利用沙鼠和TYI-S-33培养基对人和动物贾第虫属进行脱囊和培养
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