Andrews J S, Ellner J J, Stevens D P
Am J Trop Med Hyg. 1980 Jan;29(1):12-5. doi: 10.4269/ajtmh.1980.29.12.
A method is described for purification of large quantities of Giardia muris trophozoites from the small intestine of the mouse by using density layer centrifugation and nylon fiber columns. Centrifugation of trophozoites obtained from the small intestine on Metrizamide, specific gravity 1.10, followed by incubation on a nylon fiber column at 37 degrees C for 120 min, yielded up to 15 X 10(6) viable purified trophozoites per infected mouse.
描述了一种通过密度梯度离心和尼龙纤维柱从小鼠小肠中纯化大量鼠贾第虫滋养体的方法。将从小肠获得的滋养体在比重为1.10的甲泛葡胺上进行离心，然后在37℃下于尼龙纤维柱上孵育120分钟，每只感染小鼠可产生多达15×10⁶个存活的纯化滋养体。
