Addis Alessandro, Canciani Elena, Campagnol Marino, Colombo Matteo, Frigerio Christian, Recupero Daniele, Dellavia Claudia, Morroni Marco
CRABCC Animal Lab, Biotechnology Research Center for Cardiothoracic Applications, Rivolta d'Adda, 26027 Cremona, Italy.
Department of Biomedical Surgical and Dental Sciences, Università degli Studi di Milano, 20122 Milan, Italy.
Materials (Basel). 2022 Jan 28;15(3):1031. doi: 10.3390/ma15031031.
Different xenogeneic inorganic bone substitutes are currently used as bone grafting materials in oral and maxillo-facial surgery. The aim of the present study was to determine the physicochemical properties and the in vivo performance of an anorganic equine bone (AEB) substitute. AEB is manufactured by applying a process involving heating at >300 °C with the aim of removing all the antigens and the organic components. AEB was structurally characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), X-ray fluorescence (XRF), and Fourier-transformed infrared (FT-IR) spectroscopy and compared to the anorganic bovine bone (ABB). In order to provide a preliminary evaluation of the in vivo performance of AEB, 18 bone defects were prepared and grafted with AEB (nine sites), or ABB (nine sites) used as a control, in nine Yucatan Minipigs. De novo bone formation, residual bone substitute, as well as local inflammatory and tissue effects were histologically evaluated at 30 and 90 days after implantation. The structural characterization showed that the surface morphology, particle size, chemical composition, and crystalline structure of AEB were similar to cancellous human bone. The histological examination of AEB showed a comparable pattern of newly formed bone and residual biomaterial to that of ABB. Overall, the structural data and pre-clinical evidence reported in the present study suggests that AEB can be effectively used as bone grafting material in oral surgery procedures.
目前，不同的异种无机骨替代物被用作口腔颌面外科的骨移植材料。本研究的目的是确定一种无机马骨（AEB）替代物的物理化学性质和体内性能。AEB是通过在>300°C下加热的工艺制造的，目的是去除所有抗原和有机成分。通过扫描电子显微镜（SEM）、X射线衍射（XRD）、X射线荧光（XRF）和傅里叶变换红外（FT-IR）光谱对AEB进行结构表征，并与无机牛骨（ABB）进行比较。为了对AEB的体内性能进行初步评估，在9只尤卡坦小型猪身上制备了18个骨缺损，并分别用AEB（9个部位）或作为对照的ABB（9个部位）进行移植。在植入后30天和90天对新生骨形成、残余骨替代物以及局部炎症和组织效应进行组织学评估。结构表征表明，AEB的表面形态、颗粒大小、化学成分和晶体结构与松质人骨相似。AEB的组织学检查显示，新形成的骨和残余生物材料的模式与ABB相当。总体而言，本研究报告的结构数据和临床前证据表明，AEB可有效地用作口腔外科手术中的骨移植材料。
