School of Health and Life Sciences, Post-Graduate Program in Dentistry, Pontifical Catholic University of Rio Grande do Sul, Porto Alegre, RS, Brazil.
Experimental Cardiology Center, Institute of Cardiology of Rio Grande do Sul, Porto Alegre, RS, Brazil.
Clin Oral Investig. 2023 May;27(5):1907-1922. doi: 10.1007/s00784-022-04840-z. Epub 2022 Dec 27.
Considering the chemical and structural properties of dentin, this study was aimed at evaluating the effect of dentin matrix alone or combined with mesenchymal stromal cells (MSC) on postextraction alveolar bone regeneration.
Wistar rats were subjected to tooth extraction with osteotomy and allocated into groups according to the graft inserted: (1) Gelita-Spon, (2) Bio-Oss, (3) Dentin, (4) MSC, (5) Dentin/MSC, and (6) Control. Maxillae were analyzed by means of hematoxylin and eosin (H&E) staining, immunohistochemical (IHC) analysis, microcomputed tomography (micro-CT), and scanning electron microscopy (SEM). Serum levels of calcium and phosphorus were quantified.
The Bio-Oss group showed less bone than Gelita-Spon and Dentin/MSC; no other significant differences were seen in H&E analysis. The Bio-Oss group showed higher expression of collagen type I compared to the Dentin and Dentin/MSC groups and also higher osteocalcin expression than the Dentin/MSC group. There was a tendency of higher expression of osteopontin in the MSC, Dentin, and Dentin/MSC groups and higher VEGF in the MSC group. On micro-CT analysis, the Bio-Oss and the Dentin/MSC groups exhibited greater bone volume than the Control. Serum calcium and phosphorus levels did not significantly differ between the groups. SEM analysis depicted particles of Bio-Oss and dentin in the respective groups, as well as significant cellularity in the MSC group.
Autogenous nondemineralized dentin is an alternative for alveolar bone grafting, which can be improved by combination with MSC.
This work provides support for the clinical applicability of dentin graft alone or combined with MSC.
鉴于牙本质的化学和结构特性，本研究旨在评估牙本质基质单独或联合间充质基质细胞（MSC）对拔牙后牙槽骨再生的影响。
Wistar 大鼠进行截骨拔牙，并根据植入物分为以下几组：（1）Gelita-Spon，（2）Bio-Oss，（3）牙本质，（4）MSC，（5）牙本质/MSC，（6）对照组。通过苏木精和伊红（H&E）染色、免疫组织化学（IHC）分析、微计算机断层扫描（micro-CT）和扫描电子显微镜（SEM）分析上颌。定量测定血清钙、磷水平。
Bio-Oss 组的骨量少于 Gelita-Spon 和牙本质/MSC 组；H&E 分析未见其他显著差异。Bio-Oss 组的 I 型胶原表达高于牙本质和牙本质/MSC 组，骨钙素表达也高于牙本质/MSC 组。MSC、牙本质和牙本质/MSC 组的骨桥蛋白表达呈上升趋势，MSC 组的 VEGF 表达较高。在 micro-CT 分析中，Bio-Oss 和牙本质/MSC 组的骨量比对照组大。各组血清钙、磷水平无显著差异。SEM 分析显示，Bio-Oss 和牙本质组有相应的颗粒，MSC 组有明显的细胞活力。
自体非脱矿牙本质是牙槽骨移植的一种替代方法，与 MSC 联合使用可提高其效果。
本研究为牙本质单独或联合 MSC 移植的临床应用提供了支持。
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