Kim Gayeon, Rim Yeonggil, Cho Hyunwoo, Hyun Tae Kyung
Department of Industrial Plant Science and Technology, Chungbuk National University, Cheongju 28644, Korea.
Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju 52828, Korea.
Plants (Basel). 2022 Jan 26;11(3):325. doi: 10.3390/plants11030325.
roots have been used as a foodstuff and traditional medicine for thousands of years in East Asia. In order to increase the root development of . , cultivators removed the inflorescences, suggesting the possible negative effect of flowering on root development. This indicates that the genetic improvement of . by late flowering is a potential approach to increase productivity. However, nothing is known about key genes integrating multiple flowering pathways in . . In order to fill this gap, we identified potential homologs of the () gene in . . The alignment with other FT members and phylogenetic analysis revealed that the . FT (PlgFT) protein contains highly conserved functional domains and belongs to the FT-like clade. The expression analysis revealed spatial variations in the transcription of in different organs. In addition, the expression level of was increased by high temperature but not by photoperiodic light input signals, presumably due to lacking the CONSTANS binding motif in its promoter region. Furthermore, PlgFT induced early flowering upon its overexpression in . , suggesting the functional role of PlgFT in flowering. Taken together, we functionally characterized PlgFT as a master regulator of . flowering under inductive high temperature, which will serve as an important target gene for improving the root productivity.
在东亚,数千年来,根一直被用作食物和传统药物。为了增加[植物名称]的根系发育,种植者去除了花序,这表明开花可能对根系发育有负面影响。这表明通过晚花对[植物名称]进行遗传改良是提高生产力的一种潜在方法。然而,对于整合[植物名称]中多种开花途径的关键基因,我们一无所知。为了填补这一空白,我们在[植物名称]中鉴定了[FT基因名称]基因的潜在同源物。与其他FT成员的比对和系统发育分析表明,[植物名称]的FT(PlgFT)蛋白含有高度保守的功能结构域,属于FT-like进化枝。表达分析揭示了[PlgFT基因名称]在不同器官中转录的空间差异。此外,[PlgFT基因名称]的表达水平因高温而升高,但不受光周期光输入信号的影响,这可能是由于其启动子区域缺乏CONSTANS结合基序。此外,PlgFT在[植物名称]中过表达时会诱导早花,这表明PlgFT在开花中具有功能作用。综上所述,我们在功能上确定PlgFT是[植物名称]在诱导高温下开花的主要调节因子,这将作为提高根系生产力的重要靶基因。