VvFT和VvMADS8是开花整合因子FT和SOC1在葡萄中的同源基因，在葡萄中具有独特的表达模式，并能加速拟南芥开花。

VvFT and VvMADS8, the grapevine homologues of the floral integrators FT and SOC1, have unique expression patterns in grapevine and hasten flowering in Arabidopsis.

作者信息

Sreekantan Lekha, Thomas Mark R

机构信息

CSIRO Plant Industry, PO Box 350, Glen Osmond, SA 5064, Australia and Cooperative Research Centre for Viticulture, PO Box 145, Glen Osmond, SA 5064, Australia.

出版信息

Funct Plant Biol. 2006 Dec;33(12):1129-1139. doi: 10.1071/FP06144.

DOI:10.1071/FP06144

Abstract

The Vitis vinifera L. flowering genes VvFT and VvMADS8 from the grapevine cultivar Cabernet Sauvignon have been isolated. Sequence analyses showed that VvFT and VvMADS8 were highly homologous to the floral integrators, FLOWERING LOCUS T (FT) and SUPRESSOR OF OVEREXPRESSION OF CONSTANS1 (SOC1), respectively, from Arabidopsis. The expression pattern studied through real-time PCR revealed that expression of VvFT was quite low in axillary buds and high in developing inflorescence and berries. VvMADS8 was highly expressed in the axillary buds at the time when inflorescence primordia were being initiated in these buds suggesting that VvMADS8 is a gene involved in the early stages of inflorescence development. In tendrils, considered to be modified inflorescences, VvMADS8 was weakly expressed but high expression of VvFT in these organs showed that expression was irrespective of the flowering process. Through in situ hybridisation, strong expression of VvFT was detected in stamens and the ovary and ovule suggesting additional roles for VvFT in fruit and seed development. Strong expression of VvMADS8 was detected on the inflorescence primordium within the axillary bud. Overexpression of VvFT and VvMADS8 in Arabidopsis hastened flowering showing that both these genes function as promoters of flowering when ectopically expressed in a heterologous plant.

摘要

已从赤霞珠葡萄品种中分离出葡萄（Vitis vinifera L.）的开花基因VvFT和VvMADS8。序列分析表明，VvFT和VvMADS8分别与拟南芥的开花整合因子成花素基因（FLOWERING LOCUS T，FT）和CONSTANS1过表达抑制因子（SUPRESSOR OF OVEREXPRESSION OF CONSTANS1，SOC1）高度同源。通过实时PCR研究的表达模式显示，VvFT在腋芽中的表达相当低，而在发育中的花序和浆果中表达较高。VvMADS8在腋芽中花序原基开始形成时在腋芽中高表达，这表明VvMADS8是一个参与花序发育早期阶段的基因。在被认为是变态花序的卷须中，VvMADS8表达较弱，但VvFT在这些器官中高表达，表明其表达与开花过程无关。通过原位杂交，在雄蕊、子房和胚珠中检测到VvFT的强表达，这表明VvFT在果实和种子发育中还有其他作用。在腋芽内的花序原基上检测到VvMADS8的强表达。VvFT和VvMADS8在拟南芥中的过表达加速了开花，这表明这两个基因在异源植物中异位表达时均作为开花促进因子发挥作用。