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鉴定和表达分析 家族基因在 中。

Identification and expression analysis of family genes in .

机构信息

Pharmacy of College, Heilongjiang University of Chinese Medicine, Harbin, China.

School of Forestry,Northeast Forestry University, Harbin China.

出版信息

Plant Signal Behav. 2023 Dec 31;18(1):2163069. doi: 10.1080/15592324.2022.2163069.

Abstract

set ornamental, edible, and medicinal plant with broad prospects for further application development. However, there are no reports on the YABBY transcription factor in . Identification and analysis of the gene family of using bioinformatics means. Six genes were identified and divided into five subgroups. Transcriptome data and qRT-PCR were used to analyze the expression patterns of genes exhibited organ-specific patterns in expression in . Upon salt stress and drought induction, presented different morphological and physiological changes with some dynamic changes. Under salt treatment, the gene family was down-regulated; was up-regulated in leaves at 24 h. In drought treatment, , and were down-regulated to varying degrees, but was significantly up-regulated in the roots. was up-regulated gradually after being down-regulated. was significantly up-regulated in stem and leaf at 48 h. was down-regulated at first and then significantly up-regulated. The dynamic changes of salt stress and drought stress can be regarded as the responses of plants to resist damage. During the whole process of salt and drought stress treatment, the protein content of each tissue part of changed continuously. At the same time, we found that the promoter region of the gene contains stress-resistant elements, and the regulatory role of YABBY transcription factor in the anti-stress mechanism of remains to be studied. , and may be involved in the regulation of saponins in may be involved in the drought resistance mechanism in stems and leaves. This study may provide a theoretical basis for studying the regulation of terpenoids by the YABBY transcription factor and its resistance to abiotic stress.

摘要

具有观赏、食用和药用价值,具有进一步应用开发的广阔前景。然而,关于 YABBY 转录因子在 中的研究尚未见报道。本研究采用生物信息学方法对 基因家族进行鉴定和分析。共鉴定出 6 个 基因,并分为 5 个亚组。利用转录组数据和 qRT-PCR 分析 基因的表达模式,结果表明 基因在 中呈现器官特异性表达模式。在盐胁迫和干旱诱导下, 呈现出不同的形态和生理变化,具有一定的动态变化。在盐处理下, 基因家族下调表达;在叶片中 24 h 时上调表达。在干旱处理下, 、 、 不同程度下调,但 根中显著上调。 下调后逐渐上调。 茎和叶在 48 h 时显著上调。 先下调后显著上调。盐胁迫和干旱胁迫的动态变化可视为植物抵抗损伤的反应。在盐和干旱胁迫处理的整个过程中, 各组织部分的蛋白质含量不断变化。同时,我们发现 基因启动子区域含有抗逆元件,YABBY 转录因子在 抗逆机制中的调控作用有待进一步研究。 、 、 可能参与 中皂苷的调节, 可能参与 茎和叶的抗旱机制。本研究可为研究 YABBY 转录因子对萜类物质的调节及其抗非生物胁迫的机制提供理论依据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad3b/9870009/92977468b673/KPSB_A_2163069_F0001_OC.jpg

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