Amina Musarat, Al Musayeib Nawal M, Alarfaj Nawal A, El-Tohamy Maha F, Al-Hamoud Gadah A, Alqenaei Muneerah K M
Department of Pharmacognosy, Pharmacy College, King Saud University, Riyadh 11451, Saudi Arabia.
Department of Chemistry, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.
Plants (Basel). 2022 Jan 28;11(3):361. doi: 10.3390/plants11030361.
A facile, eco-friendly fluorescence approach based on the biogenic formation of zinc oxide nanoparticles using the biomass of shoots was developed. The suggested approach was employed to analyze three phenolic compounds (catechin, curviflorside, and curviflorin) isolated from the shoots of . The surface morphology of the prepared ZnONPs was characterized by carrying out different microscopic and spectroscopic investigations. A significant UV-Vis absorption peak of ZnONPs was recognized at 345 nm and the FT-IR spectra of the isolated catechin, curviflorside, and curviflorin in the presence of sodium dodecyl sulfate (SDS) and ZnONPs were recorded at λem 470, 490, and 484 nm after excitation at λex 380, 420, and 410 nm. The suggested fluorescence method displayed linear concentration ranges of 10-120, 5-100, and 10-150 μg mL for the three isolated compounds, respectively. The shoot extract, isolated compounds, and ZnONPs were screened for antibacterial and anticancer effects against four different types of bacterial strains and HeLa cells, respectively. The ZnONPs exhibited the highest zone of inhibition against and strains when compared with pure, isolated compounds and shoot extract. The anticancer potential of ZnONPs (64%) was stronger as compared to the 160 µg mL of shoot extract (49%), catechin (52%), curviflorside (54%), and curviflorin (58%) at 160 µg mL. Moreover, all the samples were investigated for hemolysis activity and showed a potent anti-hemolytic effect. The developed analytical method showed excellent sensitivity and reliability for the concurrent analysis of the isolated bioactive markers.
开发了一种简便、环保的荧光方法，该方法基于利用嫩枝生物质生物合成氧化锌纳米颗粒。所建议的方法用于分析从嫩枝中分离出的三种酚类化合物（儿茶素、弯花苷和弯花素）。通过进行不同的显微镜和光谱研究对制备的ZnONPs的表面形态进行了表征。在345nm处识别出ZnONPs的一个显著紫外可见吸收峰，在分别于380、420和410nm激发后，在470、490和484nm处记录了在十二烷基硫酸钠（SDS）和ZnONPs存在下分离出的儿茶素、弯花苷和弯花素的傅里叶变换红外光谱。所建议的荧光方法对三种分离出的化合物分别显示出10 - 120、5 - 100和10 - 150μg/mL的线性浓度范围。分别针对四种不同类型的细菌菌株和HeLa细胞对嫩枝提取物、分离出的化合物和ZnONPs进行了抗菌和抗癌作用筛选。与纯的、分离出的化合物和嫩枝提取物相比，ZnONPs对[具体细菌菌株名称缺失]和[具体细菌菌株名称缺失]菌株表现出最高的抑制圈。与160μg/mL的嫩枝提取物（49%）、儿茶素（52%）、弯花苷（54%）和弯花素（58%）相比，ZnONPs在160μg/mL时的抗癌潜力更强（64%）。此外，对所有样品进行了溶血活性研究，结果显示出有效的抗溶血作用。所开发的分析方法对分离出的生物活性标记物的同时分析显示出优异的灵敏度和可靠性。
