Department of Orthopedics, Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou 215000, PR China.
Department of Orthopedics, Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou 215000, PR China.
J Photochem Photobiol B. 2019 Jun;195:12-16. doi: 10.1016/j.jphotobiol.2019.04.001. Epub 2019 Apr 2.
The present work showed the green, eco-friendly synthesis of zinc oxide nanoparticles (ZnO NPs) using leaf extract of Cucurbita pepo. The prepared ZnO NPs were studied using different characterization techniques such as Fourier transform infrared (FTIR), X-ray diffraction (XRD), UV-Visible spectroscopy (UV-Vis) and Transmission electron microscopy (TEM). TEM images confirmed the formation of NPs with average size of 8 nm. XRD showed the crystalline nature of prepared ZnO NPs. This shows that the ZnO NPs induced cytotoxicity that affected the proliferation of MG63 osteoblast-like cells in a concentration dependent manner. Further, the ZnO NPs induced reduction in cell proliferation was confirmed by Fluorescein diacetate hydrolysis (FDA) staining. FDA staining results showed a reduction in fluorescence intensity with an increase in the concentration of ZnO NPs. The untreated control which was unaffected showed the highest fluorescence compared to ZnO NPs treated samples. A significantly diminished fluorescence was observed in samples incubated with 80 ppm ZnO NPs. In conclusion, these cell experimental results confirmed that the fabricated ZnO NPs are an important substitute for osteoporotic and bone tissue formation.
本工作展示了使用南瓜叶提取物绿色环保合成氧化锌纳米粒子（ZnO NPs）的方法。所制备的 ZnO NPs 采用傅里叶变换红外（FTIR）、X 射线衍射（XRD）、紫外可见分光光度法（UV-Vis）和透射电子显微镜（TEM）等不同的表征技术进行了研究。TEM 图像证实了 NPs 的形成，平均尺寸为 8nm。XRD 显示了所制备 ZnO NPs 的结晶性质。这表明 ZnO NPs 诱导了细胞毒性，以浓度依赖的方式影响 MG63 成骨样细胞的增殖。此外，通过荧光素二乙酸酯水解（FDA）染色证实了 ZnO NPs 诱导的细胞增殖减少。FDA 染色结果表明，随着 ZnO NPs 浓度的增加，荧光强度降低。未受处理的对照样品显示出最高的荧光强度，与 ZnO NPs 处理的样品相比。在用 80ppm ZnO NPs 孵育的样品中观察到明显减少的荧光。总之，这些细胞实验结果证实了所制备的 ZnO NPs 是治疗骨质疏松症和促进骨组织形成的重要替代物。
