Montalván Mayra, Peñafiel Manuel Alejandro, Ramírez Jorge, Cumbicus Nixon, Bec Nicole, Larroque Christian, Bicchi Carlo, Gilardoni Gianluca
Departamento de Química y Ciencias Exactas, Universidad Técnica Particular de Loja (UTPL), Calle M. Champagnat s/n, Loja 1101608, Ecuador.
Institute for Regenerative Medicine and Biotherapy (IRBM), Centre Hospitalier Universitaire de Montpellier, Inserm U1183, 34295 Montpellier, France.
Plants (Basel). 2019 Nov 15;8(11):511. doi: 10.3390/plants8110511.
The essential oils of and , belonging to the Myrtaceae family, were obtained by steam distillation. They were analyzed by gas chromatography-mass spectrometry (GC-MS), gas chromatography-flame ionization detector (GC-FID), enantioselective gas chromatography, and gas chromatography-olfactometry (GC-O). A total of 58 compounds for essential oil (EO) and 22 compounds for EO were identified and quantified by GC-MS with apolar and polar columns (including undetermined components). Major compounds (>5.0%) were limonene (5.3%-5.2%), 1,8-cineole (10.4%-11.6%), ()-caryophyllene (16.6%-16.8%), -calamenene (15.9%-14.6%), and spathulenol (6.2%-6.5%). The enantiomeric excess of eight chiral constituents was determined, being (+)-limonene and (+)-germacrene D enantiomerically pure. Eight components were identified as determinant in the aromatic profile: α-pinene, β-pinene, (+)-limonene, γ-terpinene, terpinolene, linalool, β-elemene and spathulenol. For , the major compounds (>5.0%) were α-pinene (29.2%-27.7%), β-pinene (31.3%-30.0%), myrcene (5.0%-5.2%), 1,8-cineole (8.5%-8.7%), and linalool (7.7%-8.2%). The enantiomeric excess of five chiral constituents was determined, with ()-α-pinene and (+)-germacrene D enantiomerically pure. The metabolites β-pinene, 1,8-cineole, γ-terpinene, terpinolene, linalool, and ()-β-caryophyllene were mainly responsible for the aroma of the EO. Finally, the essential oil has an inhibitory activity for cholinesterase enzymes (IC of 78.6 μg/ml and 18.4 μg/ml vs. acethylcholinesterase (AChE) and butyrylcholinesterase (BChE) respectively). This activity is of interest to treat Alzheimer's disease.
桃金娘科的[植物名称1]和[植物名称2]的精油通过水蒸气蒸馏法获得。采用气相色谱 - 质谱联用仪（GC - MS）、气相色谱 - 火焰离子化检测器（GC - FID）、对映体选择性气相色谱法和气相色谱 - 嗅觉测量法（GC - O）对其进行分析。使用非极性和极性柱（包括未确定的成分）通过GC - MS共鉴定并定量了[植物名称1]精油（EO）中的58种化合物和[植物名称2]EO中的22种化合物。主要化合物（>5.0%）为柠檬烯（5.3% - 5.2%）、1,8 - 桉叶素（10.4% - 11.6%）、（）-石竹烯（16.6% - 16.8%）、β - 卡拉烯（15.9% - 14.6%）和斯巴醇（6.2% - 6.5%）。测定了8种手性成分的对映体过量，其中（+）-柠檬烯和（+）-吉马烯D对映体纯。8种成分被鉴定为香气特征的决定性成分：α - 蒎烯、β - 蒎烯、（+）-柠檬烯、γ - 萜品烯、萜品油烯、芳樟醇、β - 榄香烯和斯巴醇。对于[植物名称2]，主要化合物（>5.0%）为α - 蒎烯（29.2% - 27.7%）、β - 蒎烯（31.3% - 30.0%）、月桂烯（5.0% - 5.2%）、1,8 - 桉叶素（8.5% - 8.7%）和芳樟醇（7.7% - 8.2%）。测定了5种手性成分的对映体过量，其中（）-α - 蒎烯和（+）-吉马烯D对映体纯。代谢产物β - 蒎烯、1,8 - 桉叶素、γ - 萜品烯、萜品油烯、芳樟醇和（）-β - 石竹烯是EO香气的主要来源。最后，[植物名称1]精油对胆碱酯酶具有抑制活性（对乙酰胆碱酯酶（AChE）和丁酰胆碱酯酶（BChE）的IC分别为78.6μg/ml和18.4μg/ml）。这种活性对于治疗阿尔茨海默病具有重要意义。
