Murai Hirotomo, Mochizuki Tomofumi
Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1, Gakuen-cho, Naka-ku, Sakai 599-8531, Japan.
Plants (Basel). 2022 Feb 4;11(3):430. doi: 10.3390/plants11030430.
is one of the most economically important flowers globally due to its high ornamental value. In recent years, a large percentage of the genome has been determined, making this species useful as a model chrysanthemum plant. To fully utilize the genome's information, efficient and rapid gene functional analysis methods are needed. In this study, we optimized the tomato aspermy virus (TAV) vector for virus-induced gene silencing (VIGS) in . Conventional plant virus inoculation methods, such as the mechanical inoculation of viral RNA transcripts and agroinoculation into leaves, did not achieve successful TAV infections in , but vacuum infiltration into sprouts was successful without symptoms. The TAV vector harboring 100 nucleotides of the () gene caused photobleaching phenotypes and a reduction in expression in . To our knowledge, this is the first report of VIGS in chrysanthemums.
由于其高观赏价值，它是全球经济上最重要的花卉之一。近年来，该物种的大部分基因组已被确定，使其成为一种有用的菊花模式植物。为了充分利用基因组信息，需要高效快速的基因功能分析方法。在本研究中，我们优化了番茄不孕病毒（TAV）载体，用于菊花中的病毒诱导基因沉默（VIGS）。传统的植物病毒接种方法，如机械接种病毒RNA转录本和农杆菌接种到叶片中，在菊花中未能成功实现TAV感染，但对芽进行真空渗透成功且无病症。携带100个核苷酸的（）基因的TAV载体在菊花中引起了光漂白表型并降低了表达。据我们所知，这是菊花中VIGS的首次报道。
