Yang Qiyu, Fan Youwei, Luo Shuwen, Liu Chun, Yuan Suxia
State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Plants (Basel). 2024 Dec 3;13(23):3396. doi: 10.3390/plants13233396.
, renowned for its large inflorescences and a diverse range of colors, highlights a significant limitation in current gene function research, which is the lack of effective molecular genetic tools. This study utilized a (TRV)-based virus-induced gene silencing (VIGS) system to investigate gene function through posttranscriptional gene silencing in for the first time. The ortholog of () in , termed , was identified. Infection of tissue-cultured seedlings with TRV- led to photobleaching of the leaves. Additionally, infection with TRV containing the fragment in the flowers resulted in decreased anthocyanin production in sepals and a lightening of sepal coloration in the infected flowers. The phenomena and RT-qPCR results proved that the and genes of hydrangea were successfully silenced via the vacuum infiltration method. Furthermore, the introduction of TRV- revealed a decrease in delphinidin-3-glucoside content in sepals and caused a color change in the sepals from blue to pink. This study demonstrated that the TRV-VIGS system was successfully established in and effectively applied to the function analysis of .
因其巨大的花序和多样的颜色而闻名，凸显了当前基因功能研究中的一个重大局限，即缺乏有效的分子遗传工具。本研究首次利用基于烟草脆裂病毒（TRV）的病毒诱导基因沉默（VIGS）系统，通过转录后基因沉默来研究绣球花中的基因功能。在绣球花中鉴定出了拟南芥中AtPAP1的直系同源基因，命名为HhPAP1。用携带HhPAP1的TRV感染组织培养的幼苗导致叶片光漂白。此外，在花中用携带HhPAP1片段的TRV感染导致萼片中花青素产量降低，受感染花朵的萼片颜色变浅。这些现象和RT-qPCR结果证明，绣球花的HhPAP1和HhMYB113基因通过真空渗透法成功沉默。此外，引入携带HhF3′5′H的TRV显示萼片中飞燕草素-3-葡萄糖苷含量降低，并导致萼片颜色从蓝色变为粉红色。本研究表明，TRV-VIGS系统在绣球花中成功建立，并有效地应用于HhPAP1的功能分析。
