Matsumoto K, Natori T, Agui T, Tsutimoto S, Matsuhashi A, Gasser D L
Biochem Genet. 1986 Feb;24(1-2):93-102. doi: 10.1007/BF00502981.
Rat serum was fractionated on a column of Sephacryl-300 and tested with a rabbit anti-rat beta-2-microglobulin (B2m) antiserum. This antiserum was directed against B2m purified from rat liver, and its specificity was confirmed by immunoprecipitation procedures. The antiserum recognized three peaks in the fractionated rat serum: a 200- to 300-kd (kilodalton) fraction, a 40- to 70-kd component, and the free 12-kd B2m. Indirect immunoprecipitation from the 200- to 300-kd fraction led to the identification of a 43-kd polypeptide associated with B2m. A xenoantiserum against RT1 class I antigen also precipitated a similar polypeptide from the same fraction, but this molecule differed in size and antigenic specificity from the one precipitated by anti-rat B2m.
将大鼠血清在Sephacryl - 300柱上进行分级分离，并用兔抗大鼠β2 - 微球蛋白（B2m）抗血清进行检测。该抗血清针对从大鼠肝脏纯化的B2m，其特异性通过免疫沉淀程序得以证实。该抗血清在分级分离的大鼠血清中识别出三个峰：一个200至300千道尔顿（kd）的级分、一个40至70 kd的组分以及游离的12 kd B2m。从200至300 kd级分进行间接免疫沉淀，鉴定出一种与B2m相关的43 kd多肽。针对RT1 I类抗原的异种抗血清也从同一级分中沉淀出一种类似的多肽，但该分子在大小和抗原特异性上与抗大鼠B2m沉淀出的分子不同。
