Department of Life Science, Hanyang University, Seoul 04763, Korea.
Research Institute for Convergence of Basic Sciences, Hanyang University, Seoul 04763, Korea.
Int J Mol Sci. 2022 Jan 30;23(3):1605. doi: 10.3390/ijms23031605.
Activity-based monitoring of cell-secreted proteases has gained significant interest due to the implication of these substances in diverse cellular functions. Here, we demonstrated a cell-based method of monitoring protease activity using fluorescent cell-permeable peptides. The activatable peptide consists of anionic (EEEE), cleavable, and cationic sequences (RRRR) that enable intracellular delivery by matrix metalloproteinase-2 (MMP2), which is secreted by living cancer cells. Compared to HT-29 cells (MMP2-negative), HT-1080 cells (MMP2-positive) showed a strong fluorescence response to the short fluorescent peptide via cell-secreted protease activation. Our approach is expected to find applications for the rapid visualization of protease activity in living cells.
由于这些物质在多种细胞功能中的重要作用，基于细胞活动的细胞分泌蛋白酶监测已引起广泛关注。在此，我们展示了一种使用荧光细胞可渗透肽监测蛋白酶活性的基于细胞的方法。该激活肽由阴离子（EEEE）、可切割和阳离子序列（RRRR）组成，可通过基质金属蛋白酶-2（MMP2）进行细胞内递送至活癌细胞，而 MMP2 由活癌细胞分泌。与 HT-29 细胞（MMP2 阴性）相比，HT-1080 细胞（MMP2 阳性）通过细胞分泌的蛋白酶激活表现出对短荧光肽的强烈荧光响应。我们的方法有望快速可视化活细胞中的蛋白酶活性。
Zotero 插件
