Department of Chemistry and Food Toxicology, Institute of Food Technology and Nutrition, College of Natural Sciences, University of Rzeszow, 35-601 Rzeszow, Poland.
Department of Biotechnology and Bioinformatics, Faculty of Chemistry, Rzeszow University of Technology, 35-959 Rzeszow, Poland.
Molecules. 2022 Jan 22;27(3):720. doi: 10.3390/molecules27030720.
Fir honeydew honey is a uniquely beneficial product which is often subjected to adulteration; however, pollen analysis is not useful to verify this honey type. Fourteen samples of EU protected designation of origin fir honeydew honey gathered directly from apiaries were studied. Standards of legal requirements and additional parameters, i.e., specific optical rotation, mineral content, and antioxidant activity, were tested. Five nectar honeys of different varieties were used as a comparative material. HPTLC and SDS-PAGE methods were used to fingerprint the honey types. All honeys tested fulfilled the quality requirements in terms of water content, pH, total acidity, conductivity, HMF, and diastase number. They were defined as dark amber on the Pfund scale and exhibited positive specific rotation (+2.5 to 25). Honeydew honey surpassed the tested nectar honeys in terms of mineral content and antioxidant activity as well as total polyphenolic content, except for buckwheat honey. The sugar and polyphenolic profile obtained by HPTLC allowed to distinguish honeydew from nectar honeys. The same was achieved by SDS-PAGE protein profiling. Both techniques seem to be cheap and quick tools for precisely distinguishing honeydew honey.
冷杉蜜露蜜是一种独特的有益产品，经常受到掺假；然而，花粉分析对于验证这种蜂蜜类型并不有用。对直接从养蜂场采集的 14 份欧盟受保护原产地名称冷杉蜜露蜜进行了研究。测试了法定要求的标准和其他参数，即特定旋光度、矿物质含量和抗氧化活性。使用了五种不同品种的花蜜蜂蜜作为比较材料。使用 HPTLC 和 SDS-PAGE 方法对蜂蜜类型进行指纹识别。所有测试的蜂蜜在水分含量、pH 值、总酸度、电导率、糠醛和淀粉酶数方面均符合质量要求。根据 Pfund 量表，它们被定义为深琥珀色，并具有正的特定旋光度（+2.5 至 25）。与测试的花蜜蜂蜜相比，蜜露蜂蜜在矿物质含量和抗氧化活性以及总多酚含量方面都更优，除了荞麦蜜。通过 HPTLC 获得的糖和多酚图谱可以将蜜露与花蜜蜂蜜区分开来。SDS-PAGE 蛋白图谱也达到了同样的效果。这两种技术似乎都是廉价且快速的工具，可以准确地区分蜜露蜂蜜。
