Nadon Sudarut, Leksawasdi Noppol, Jantanasakulwong Kittisak, Rachtanapun Pornchai, Ruksiriwanich Warintorn, Sommano Sarana Rose, Khaneghah Amin Mousavi, Castagnini Juan M, Barba Francisco J, Phimolsiripol Yuthana
Faculty of Agro-Industry, Chiang Mai University, Chiang Mai 50100, Thailand.
Cluster of Agro Bio-Circular-Green Industry, Chiang Mai University, Chiang Mai 50100, Thailand.
Plants (Basel). 2023 Jun 3;12(11):2211. doi: 10.3390/plants12112211.
This research aimed to optimize pressure (10-20 MPa) and temperature (45-60 °C) conditions for supercritical fluid extraction (SFE) of Makwaen pepper () extract (ME) in comparison to conventional hydro-distillation extraction. Various quality parameters, including yield, total phenolic compounds, antioxidants, and antimicrobial activities of the extracts, were assessed and optimized using a central composite design. The optimal SFE conditions were found to be 20 MPa at 60 °C, which resulted in the highest yield (19%) and a total phenolic compound content of 31.54 mg GAE/mL extract. IC values for DPPH and ABTS assays were determined to be 26.06 and 19.90 μg/mL extract, respectively. Overall, the ME obtained through SFE exhibited significantly better physicochemical and antioxidant properties compared to ME obtained through hydro-distillation extraction. Gas chromatography-mass spectrometry (GC-MS) analysis revealed that beta-pinene was the major component in the ME obtained through SFE (23.10%), followed by d-limonene, alpha-pinene, and terpinen-4-ol at concentrations of 16.08, 7.47, and 6.34%, respectively. On the other hand, the hydro-distillation-extracted ME showed stronger antimicrobial properties than the SFE-extracted ME. These findings suggest that both SFE and hydro-distillation have the potential for extracting Makwaen pepper, depending on the intended purpose of use.
本研究旨在优化超临界流体萃取（SFE）Makwaen胡椒（）提取物（ME）的压力（10 - 20兆帕）和温度（45 - 60℃）条件，并与传统水蒸馏萃取进行比较。使用中心复合设计评估并优化了提取物的各种质量参数，包括产率、总酚类化合物、抗氧化剂和抗菌活性。发现最佳SFE条件为60℃、20兆帕，此条件下产率最高（19%），提取物中总酚类化合物含量为31.54毫克没食子酸当量/毫升。DPPH和ABTS测定的IC值分别确定为26.06和19.90微克/毫升提取物。总体而言，通过SFE获得的ME与通过水蒸馏萃取获得的ME相比，具有显著更好的理化性质和抗氧化性能。气相色谱 - 质谱（GC - MS）分析表明，β-蒎烯是通过SFE获得的ME中的主要成分（23.10%），其次是d - 柠檬烯、α-蒎烯和萜品烯 - 4 - 醇，浓度分别为16.08%、7.47%和6.34%。另一方面，水蒸馏萃取的ME比SFE萃取的ME具有更强的抗菌性能。这些发现表明，根据预期用途，SFE和水蒸馏都有提取Makwaen胡椒的潜力。
