Max Delbrück Center for Molecular Medicine in the Helmholtz Association, 13125 Berlin, Germany.
Berlin Institute of Health, Metabolomics Platform, 10178 Berlin, Germany.
Molecules. 2022 Jan 25;27(3):777. doi: 10.3390/molecules27030777.
The study of the bioavailability of bioactive compounds is a fundamental step for the development of applications based on them, such as nutraceuticals, functional foods or cosmeceuticals. It is well-known that these compounds can undergo metabolic reactions before reaching therapeutic targets, which may also affect their bioactivity and possible applications. All recent studies that have focused on bioavailability and metabolism of phenolic and terpenoid compounds have been developed because of the advances in analytical chemistry and metabolomics approaches. The purpose of this review is to show the role of analytical chemistry and metabolomics in this field of knowledge. In this context, the different steps of the analytical chemistry workflow (design study, sample treatment, analytical techniques and data processing) applied in bioavailability and metabolism in vivo studies are detailed, as well as the most relevant results obtained from them.
生物活性化合物生物利用度的研究是基于它们的应用开发的基础步骤，例如营养保健品、功能性食品或化妆品。众所周知，这些化合物在到达治疗靶点之前可能会发生代谢反应，这也可能影响它们的生物活性和潜在应用。所有最近专注于酚类和萜烯类化合物生物利用度和代谢的研究都是因为分析化学和代谢组学方法的进步而发展起来的。本文的目的是展示分析化学和代谢组学在这一知识领域中的作用。在这方面，详细介绍了分析化学工作流程的不同步骤（研究设计、样品处理、分析技术和数据处理）在体内生物利用度和代谢研究中的应用，以及从中获得的最相关结果。
