Max Delbrück Center for Molecular Medicine in the Helmholtz Association, 13125 Berlin, Germany.
Berlin Institute of Health Metabolomics Platform, 10178 Berlin, Germany.
Int J Mol Sci. 2021 Mar 22;22(6):3220. doi: 10.3390/ijms22063220.
The approaches based on high-resolution analytical techniques, such as nuclear magnetic resonance or mass spectrometry coupled to chromatographic techniques, have a determining role in several of the stages necessary for the development of functional foods. The analyses of botanical extracts rich in bioactive compounds is one of the fundamental steps in order to identify and quantify their phytochemical composition. However, the compounds characterized in the extracts are not always responsible for the bioactive properties because they generally undergo metabolic reactions before reaching the therapeutic targets. For this reason, analytical techniques are also applied to analyze biological samples to know the bioavailability, pharmacokinetics and/or metabolism of the compounds ingested by animal or human models in nutritional intervention studies. In addition, these studies have also been applied to determine changes of endogenous metabolites caused by prolonged intake of compounds with bioactive potential. This review aims to describe the main types and modes of application of high-resolution analytical techniques in all these steps for functional food development.
基于高分辨率分析技术（如与色谱技术相结合的核磁共振或质谱）的方法在功能性食品开发所需的多个阶段中起着决定性作用。分析富含生物活性化合物的植物提取物是确定和定量其植物化学成分的基本步骤之一。然而，提取物中所鉴定的化合物并不总是具有生物活性特性，因为它们通常在到达治疗靶点之前会经历代谢反应。出于这个原因，分析技术也被应用于分析生物样本，以了解在营养干预研究中动物或人类模型摄入的化合物的生物利用度、药代动力学和/或代谢情况。此外，这些研究还被应用于确定由于长期摄入具有生物活性潜力的化合物而引起的内源性代谢物的变化。本文旨在描述高分辨率分析技术在功能性食品开发的所有这些步骤中的主要类型和应用模式。
