Academy of Agriculture and Forestry, Qinghai University, Xining, Qinghai 810016 China.
Key Laboratory of Potato Breeding in Qinghai Province, Xining, Qinghai 810016 China.
Bioengineered. 2022 Feb;13(2):3852-3867. doi: 10.1080/21655979.2021.2024375.
This study investigated the inhibitory activity of S61 and its active extract on potato dry rot pathogens and aimed at contributing to biocontrol agent development during potato storage. Three kinds of pathogens were isolated as target pathogenic fungi from dry rot tubers and determined as (Qing 9A-2), (Qing 9A-5-8) and (Qing 9A-1-1) by morphological and molecular identification. The strain S61 and its extract exhibited a higher inhibitory rate on both three pathogens (56.32-65.75 and 1.67-51.11%), notably the best suppression efficiency is presented in S61 and 40 mg/mL ethyl acetate extract. In terms of in vivo effects, both S61 and its ethyl acetate extract effectively reduced the decayed fruit and weight loss rate (0-20% and 7.59-16.56%) and enhanced the defensive enzymatic activities to improve resistance. In addition, strain S61 could be colonized on potato tubers, especially the highest amount of 1.55 × 10 CFU/mL on fifth day for variety Xiazhai 65. Overall, S61 and its ethyl acetate extract could be considered as potential approach for biocontrol potato dry rot.
本研究调查了 S61 及其活性提取物对马铃薯干腐病病原菌的抑制活性，旨在为马铃薯贮藏期生物防治剂的开发提供依据。从干腐薯块中分离出三种病原菌作为目标致病真菌，通过形态学和分子鉴定分别确定为（Qing 9A-2）、（Qing 9A-5-8）和（Qing 9A-1-1）。菌株 S61 及其提取物对三种病原菌均表现出较高的抑制率（56.32-65.75%和 1.67-51.11%），其中 S61 和 40mg/mL 乙酸乙酯提取物的抑制效果最好。在体内效果方面，S61 及其乙酸乙酯提取物均能有效降低腐烂果实和失重率（0-20%和 7.59-16.56%），并增强防御酶活性以提高抗性。此外，菌株 S61 可以在马铃薯块茎上定殖，特别是在第五天，品种夏寨 65 的数量最高可达 1.55×10 CFU/mL。总之，S61 及其乙酸乙酯提取物可作为防治马铃薯干腐病的潜在方法。
