Kirby J A, Pepper J R, Reader J A, Corbishley C M, Hudson L
Clin Exp Immunol. 1986 Feb;63(2):334-42.
Dogs receiving left unilateral allo- or auto-grafts were treated with Cyclosporin A (Cy A) for 4 days. Thereafter allografted transplant recipients showed pulmonary pathology consistent with rejection. Blood and bronchoalveolar lavage (BAL)-derived lymphocytes were isolated from the recipient animals before and at various times after operation and the frequency of allospecific precursor cytolytic lymphocytes (pCTL) determined by limiting dilution analysis (LDA). Samples from the autografted control animal did not show any post-operative frequency changes; however, both blood and BAL-derived lymphocytes from allografted recipients showed a significant post-operative increase in the proportion of donor-specific pCTL. This increase was consistently greater in samples from the transplanted than the autochthonous lung. The frequency of pCTL determined using targets from an unrelated dog showed no post-operative increase. It is likely that the increase in frequency of donor-specific pCTL recorded during graft-rejection is a specific consequence of an interaction between the graft and recipient's immune system.
接受左侧单侧同种异体或自体移植的犬用环孢素A（Cy A）治疗4天。此后，同种异体移植的受体出现与排斥反应一致的肺部病理变化。在手术前及术后不同时间从受体动物中分离出血液和支气管肺泡灌洗（BAL）来源的淋巴细胞，并通过有限稀释分析（LDA）确定同种异体特异性前体细胞溶解淋巴细胞（pCTL）的频率。自体移植对照动物的样本未显示术后频率变化；然而，同种异体移植受体的血液和BAL来源的淋巴细胞在术后均显示供体特异性pCTL比例显著增加。移植肺样本中的这种增加始终大于自体肺样本。使用来自无关犬的靶标确定的pCTL频率术后未增加。移植排斥期间记录的供体特异性pCTL频率增加可能是移植物与受体免疫系统相互作用的特定结果。
