Echeverria P, Taylor D N, Leksomboon U, Blacklow N R, Pinnoi S, Nataro J P, Kaper J, Rowe B
Diagn Microbiol Infect Dis. 1986 Apr;4(4):277-84. doi: 10.1016/0732-8893(86)90067-2.
Enteric pathogens were identified in children with diarrhea from duodenal specimens obtained with a string capsule and from fecal specimens. Rotavirus was identified in stools of 43 of 100 children, and was recovered from the small intestine from nine (21%) children who were excreting this virus. Shigella was isolated from stools from 22, Salmonella from 17, enterotoxigenic Escherichia coli from eight, and Aeromonas hydrophila from one of 100 children with diarrhea. In contrast to rotavirus, Salmonella, Shigella, enterotoxigenic Escherichia coli, and A. hydrophila were not isolated from the small intestine. Nonenterotoxigenic Aeromonas species were recovered from the small intestine, but not the stool of five children. These children were also infected with Shigella or with rotavirus; this suggests that Aeromonas was not the cause of their diarrhea. None of 51 Escherichia coli isolated with the string capsules, or 67 isolated from stool that agglutinated in commercial enteropathogenic Escherichia coli antisera were of classical enteropathogenic Escherichia coli serotypes. One hundred and five of these 118 Escherichia coli did not hybridize with a deoxyribonucleic acid probe for plasmid mediated factors conferring adherence to HeLa cells. Examination of specimens collected with a string capsule from children with diarrhea did not identify any more enteric pathogens than examining stools. Furthermore testing Escherichia coli for agglutination in commercial enteropathogenic Escherichia coli antisera did not identify Escherichia coli of enteropathogenic serotypes.
通过线囊获取十二指肠标本以及粪便标本，在腹泻儿童中鉴定出肠道病原体。100名儿童中有43名粪便中检出轮状病毒，其中9名（21%）排病毒儿童的小肠中也检出该病毒。100名腹泻儿童中，22名粪便中分离出志贺菌，17名分离出沙门菌，8名分离出产肠毒素大肠杆菌，1名分离出嗜水气单胞菌。与轮状病毒不同，沙门菌、志贺菌、产肠毒素大肠杆菌和嗜水气单胞菌未从小肠中分离出来。5名儿童的小肠中分离出非产肠毒素气单胞菌，但粪便中未分离出。这些儿童也感染了志贺菌或轮状病毒；这表明气单胞菌不是他们腹泻的病因。用线囊分离出的51株大肠杆菌，或从粪便中分离出并在商业性肠致病性大肠杆菌抗血清中凝集的67株大肠杆菌，均不属于经典的肠致病性大肠杆菌血清型。这118株大肠杆菌中有105株未与用于检测质粒介导的黏附HeLa细胞因子的脱氧核糖核酸探针杂交。对腹泻儿童用线囊采集的标本进行检测，发现的肠道病原体并不比检测粪便多。此外，检测大肠杆菌在商业性肠致病性大肠杆菌抗血清中的凝集情况，也未鉴定出肠致病性血清型的大肠杆菌。
