State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023, China.
Institute of Nanochemistry and Nanobiology, School of Environmental and Chemical Engineering, Shanghai University, Shanghai, 200444, China.
Angew Chem Int Ed Engl. 2022 Apr 11;61(16):e202117401. doi: 10.1002/anie.202117401. Epub 2022 Feb 23.
We report a photodynamic therapy driven by electrochemiluminescence (ECL). The luminescence generated by Ru(bpy) and co-reactant tripropylamine (TPA) pair acts as both optical readout for ECL imaging, and light source for the excitation of photosensitizer to produce reactive oxygen species (ROS) in photodynamic therapy (PDT) system. The ECL-driven PDT (ECL-PDT) relies on the effective energy transfer from ECL emission to photosensitizer chlorin e6 (Ce6), which sensitizes the surrounding O into ROS. The dynamic process of gradual morphological changes, the variation of cell-matrix adhesions, as well as the increase of cell membrane permeability in the process of ECL-PDT were monitored under ECL microscopy (ECLM) with good spatiotemporal resolution. Combining real-time imaging with ECL-PDT, this new strategy provides not only new insights into dynamic cellular processes, but also promising potential of ECL in clinical applications.
我们报告了一种电致化学发光（ECL）驱动的光动力疗法。由 Ru(bpy) 和共反应物三丙胺（TPA）对产生的发光既作为 ECL 成象的光学读出，也作为光动力治疗（PDT）系统中激发光敏剂产生活性氧物种（ROS）的光源。ECL 驱动的 PDT（ECL-PDT）依赖于 ECL 发射到光敏剂叶绿素 e6（Ce6）的有效能量转移，Ce6 将周围的 O 敏化为 ROS。在 ECL 显微镜（ECLM）下，以良好的时空分辨率监测了 ECL-PDT 过程中形态变化的逐渐动态过程、细胞-基质黏附的变化以及细胞膜通透性的增加。通过将实时成像与 ECL-PDT 相结合，这种新策略不仅为动态细胞过程提供了新的见解，而且为 ECL 在临床应用中的潜在应用提供了前景。
