State Key Laboratory of Analytical Chemistry for Life Science, Chemistry and Biomedicine Innovation Center (ChemBIC), School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, Jiangsu, 210023, China.
Institute of Analytical Chemistry, Department of Chemistry, Zhejiang University, Hangzhou, Zhejiang, 310058, China.
ChemistryOpen. 2023 May;12(5):e202200113. doi: 10.1002/open.202200113. Epub 2022 Jul 26.
Understanding biological mechanisms operating in cells is one of the major goals of biology. Since heterogeneity is the fundamental property of cellular systems, single cell measurements can provide more accurate information about the composition, dynamics, and regulatory circuits of cells than population-averaged assays. Electrochemiluminescence (ECL), the light emission triggered by electrochemical reactions, is an emerging approach for single cell analysis. Numerous analytes, ranging from small biomolecules such as glucose and cholesterol, proteins and nucleic acids to subcellular structures, have been determined in single cells by ECL, which yields new insights into cellular functions. This review aims to provide an overview of research progress on ECL principles and systems for single cell analysis in recent years. The ECL reaction mechanisms are briefly introduced, and then the advances and representative works in ECL single cell analysis are summarized. Finally, outlooks and challenges in this field are addressed.
了解细胞中生物机制的运作是生物学的主要目标之一。由于异质性是细胞系统的基本特性，单细胞测量可以比群体平均测定提供关于细胞组成、动力学和调控回路的更准确信息。电致化学发光（ECL）是电化学反应触发的发光，是单细胞分析的一种新兴方法。通过 ECL 已经在单细胞中测定了许多分析物，从小分子如葡萄糖和胆固醇、蛋白质和核酸到亚细胞结构，这为细胞功能提供了新的见解。本综述旨在概述近年来单细胞分析中 ECL 原理和系统的研究进展。简要介绍了 ECL 反应机制，然后总结了 ECL 单细胞分析的进展和代表性工作。最后，讨论了该领域的展望和挑战。
